Rpa primers

The nematodes used in this study are listed in Table 1. Two rice grain samples, AB1 and AB2, infected by A. besseyi were collected from paddy fields in Shanghai and Nanjing, respectively. The related Aphelenchoides spp., A. fragariae (AF), and A. subtenuis (AS), were intercepted in Shanghai ports and saved in Shanghai Entry-Exit Inspection and Quarantine Bureau. M. incognita (MI) were kindly provided by Prof. Xuan Wang (Nanjing Agricultural University, Nanjing, China) (Wang et al., 2018 (link)).
RPA primers, crRNA, and ssDNA reporters were synthesized by Sangon Biotech (Shanghai, China). The RPA assay kit (TwistAmp Basic kit, TABAS03KIT) was purchased from TwistDx Ltd. (TwistDx Ltd., United Kingdom). The LbCas12a (cpf1) nuclease (32108-03) was purchased from Tolo Biotech (Tolo Biotech, Anhui, China). The lateral flow strip (JY0301) was purchased from Warbio Biotech (Nanjing, China).

The following bacterial strains were available in our lab: F. tularensis, Brucella melitensis, Brucella abortus, Burkholderia pseudomallei, Burkholderia mallei, Bacillus anthracis, Staphylococcus aureus, Bacillus thuringiensis, Yersinia pestis, Salmonella typhi, Bacillus subtilis, Escherichia coli, Vibrio vulnificus, Staphylococcus epidermidis, Vibrio parahaemolyticus, Bacillus cereus, and Vibrio cholerae. RPA primers, RPA probes, crRNA, and fluorescent single-stranded DNA reporter (Flu-ssDNA) were synthesized by Shanghai Sangon Biotech Co., Ltd. (China). LbCas12a protein, NEBuffer 3.1, and RNA inhibitor were provided by New England BioLabs, Inc. (USA). TwistAmp™ Exo Kit and TwistAmp™ basic Kit were provided by TwistDx (Cambridge, UK). A positive reference plasmid for F. tularensis detection (pEASY-T1-TUL4) was constructed by our lab. DNase/RNase-free distilled, deionized water (DDH₂O) was provided by Tiangen Biochemical Co., Ltd. A QIAamp™ DNA Mini Kit (Qiagen, Germany) was used to extract bacterial strain genomes, followed by the user manual's protocol.