Uplc grade methanol

EtOAc (used for fungal extraction) was purchased from VWR International GmbH, Hannover, Germany. UPLC grade methanol, acetonitrile and water used for UPLC/MS analysis were purchased from BiosolveChimie, Dieuze, France. Formic acid (UPLC/MS optigrade) was obtained from LGC Standards Promochem©, Wesel, Germany. UPLC-QToF-MS/MS analyses were carried out on an ACQUITY UPLC I-Class System coupled to the Xevo G2-XS QToF Mass Spectrometer (Waters^®, Milford, MA, USA). Czapek broth, yeast extracts and malt extracts were purchased from BD Bioscience, Sparks, NE, USA. Agar was purchased from Applichem, Darmstadt, Germany. Peptone from soymeal and glucose were purchased from Merck, Darmstadt, Germany. Potato extract was from Sigma-Aldrich, Schnelldorf, Germany. Sucrose was purchased from Handelsmarken, Offenburg, Germany. Casein hydrolysate was purchased from Carl Roth, Karlsruhe, Germany.

1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) and n-oleoyl-d-erythro sphingosylphosphorylcholine (SM (d18:1/18:1)) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Methyl-tert-buthylether (MTBE) and chloroform were purchased from Sigma Aldrich (Taufkirchen, Germany). Potassium ferrocyanide (K₄[Fe(CN)₆]), disodium hydrogen phosphate (Na₂HPO₄), and sodium dihydrogen phosphate (NaH₂PO₄) were obtained from Merck (Darmstadt, Germany). Sodium sulfate was purchased from VWR (Darmstadt, Germany). UPLC-grade methanol, acetonitrile, formic acid, and isopropanol were obtained from Biosolve BV (Valkenswaard, Netherlands). Hydrogen peroxide (30%) and 32% ammonia solutions are from Carl Roth (Karlsruhe, Germany). 50 mM buffer solution of (Na₂HPO₄/NaH₂PO₄) (pH = 7.4) was used in the experiments. All chemicals employed were used without further purification. In all solutions and experiments ultrapure water was used (resistivity: 18.2 MΩ.cm, conductivity: 0.055 μS/cm) prepared by Thermo Scientific GenPure pro water purification system (Niederelbert, Germany).