Hydroxylamine hydrochloride h3no hcl

Manufactured by Thermo Fisher Scientific

Hydroxylamine hydrochloride (H3NO•HCl) is a chemical compound commonly used in various laboratory applications. It is a white crystalline solid that is soluble in water and other polar solvents. The primary function of hydroxylamine hydrochloride is to serve as a reducing agent in chemical reactions and analyses.

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Lab products found in correlation

Ch3cooh, by Thermo Fisher Scientific (2 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Hydroxylamine (20 citations) Hydroxylamine hydrochloride (15 citations)

2 protocols using hydroxylamine hydrochloride h3no hcl

Preparation of Buffered Acetic Acid Solutions

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Preparation of 2L of of buffered acetic acid solution (Step 4):

Begin with 1L MQ water in a 2L bottle (MQ water = Milli-Q® purified water that has resistance of 18.2 MΩ•cm at 25°C)

Add 164g sodium acetate (C2H3NaO2 Anhydrous, Fisher Chemical, Certified ACS, F.W. = 82.03, S210-2)

Add 114mL glacial acetic acid (CH3COOH, Fisher Chemical, Certified ACS, F.W.=60.05, product A38C-212)

Fill to 2L with MQ

Preparation of 500mL of buffered acetic acid/sodium acetate (pH 4) solution (Step 5):

start with 250mL MQ

Add 1.84g hydroxylamine hydrochloride (H3NO•HCl, Alfa Aesar, 99% purity, F.W. 69.49, Stock #: A15398)

Add 76mL glacial acetic acid (CH3COOH)

Add 10g NaOH pellets (Fisher Chemical, Certified ACS, F.W. = 40, Catalogue #: S318-1)

Fill to 500mL with MQ

Check pH after 1hr; should be ~4

Simon M.H., Babin D.P., Goldstein S.L., Cai M.Y., Liu T., Han X., Haws A.A., Johns M., Lear C, & Hemming S.R. (2020). Sequential extraction procedure to obtain the composition of terrigenous detritus in marine sediments. MethodsX, 7, 100888.

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Isolation and Purification of DNA

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Chemicals used in this procedure include the following:

Hydrogen peroxide 3% (H2O2, Fisher Chemical, Certified, Catalog No. H324-500)

Sodium acetate (C2H3NaO2 Anhydrous, Fisher Chemical, Certified ACS, F.W. = 82.03, S210-2)

Glacial acetic acid (CH3COOH, Fisher Chemical, Certified ACS, F.W.=60.05, product A38C-212)

Hydroxylamine hydrochloride (H3NO•HCl, Alfa Aesar, 99% purity, F.W. 69.49, Stock #: A15398)

Sodium hydroxide pellets (NaOH, Fisher Chemical, Certified ACS, F.W. = 40, Catalogue #: S318-1)

0.5% sodium metaphosphate solution ((NaPO3)6, Fischer Chemical, Laboratory Grade Powder, Catalogue #: S333-500)

Cesium chloride (CsCl, INDOFINE, Research Grade, F.W. = 168.36, Product #: MB1006)

Ethanol (C2H6O, Ethyl Alcohol Denatured (Proprietary Solvent), Certified, Fisher Chemical, F.W. = 46.069, Catalogue #: A407-500)

deionized water (Milli-Q® purified water that has resistance of 18.2 MΩ•cm at 25°C)

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