Luminex lx200

Manufactured by Merck Group

Sourced in United States

The Luminex™ (LX200) is a multi-analyte detection system designed for high-throughput quantitative analysis. It utilizes bead-based technology to simultaneously detect and quantify multiple analytes in a single sample. The core function of the Luminex™ (LX200) is to provide a platform for rapid and accurate measurement of various biomolecules, such as proteins, antibodies, and nucleic acids.

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Lab products found in correlation

Immunocap system, by Thermo Fisher Scientific (3 mentions) Milliplex map human circulating cancer biomarker magnetic bead panel 1, by Merck Group (1 mentions) Xponent v 3, by Merck Group (1 mentions) Human high sensitivity t cell magnetic bead panel kit, by Merck Group (1 mentions) Cobas 8000, by Roche (1 mentions)

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Luminex instrument (LX200; (2 citations)

4 protocols using luminex lx200

Quantifying Circulating Insulin Levels

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Circulating insulin levels were quantified in plasma samples collected during the OGTT using a MILLIPLEX™ Mouse Metabolic Hormone Panel – Metabolism Multiplex Assay (Merck) using a Luminex™ (LX200) following manufacturer’s instructions.

Debédat J., Le Roy T., Voland L., Belda E., Alili R., Adriouch S., Bel Lassen P., Kasahara K., Hutchison E., Genser L., Torres L., Gamblin C., Rouault C., Zucker J.D., Kapel N., Poitou C., Marcelin G., Rey F.E., Aron-Wisnewsky J, & Clément K. (2022). The human gut microbiota contributes to type-2 diabetes non-resolution 5-years after Roux-en-Y gastric bypass. Gut Microbes, 14(1), 2050635.

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Assessing Antipsychotic-Induced Hyperprolactinemia

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Antipsychotic-induced hyperprolactinemia is a proxy indicator of D₂ receptor occupancy manifesting when D₂ receptor occupancy is greater than 72% (96 (link)). Serum prolactin was assessed as a surrogate for central nervous system D2 receptor binding affinity for haloperidol and clozapine. Prolactin levels were determined from serum samples using the MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 1 (EMD Millipore, Burlington, MA, USA; Cat# HCCBP1MAG58K) containing prolactin coupled with the Luminex LX200 (EMD Millipore, Burlington, MA, USA) platform in a magnetic bead format according to manufacturer’s instructions. All serum samples were assayed in duplicate, and a pooled serum sample was included as a positive control. Concentrations are expressed as ng/ml. Data were analyzed using xPONENT v.3.1 software (EMD Millipore, Burlington, MA, USA). The minimum detectable concentration for prolactin was 30.2 pg/ml. The coefficient of determination for prolactin was 0.9999. The intra-assay precision was 4.9–15% and the inter-assay precision 4.1–16.2%. Intra-assay precision is generated from the mean of the %CV’s from 16 reportable results across two different concentrations of analytes in a single assay. Inter-assay precision is generated from the mean of the %CV’s across two different concentrations of analytes across 10 different assays.

Hemby S.E, & McIntosh S. (2023). Chronic haloperidol administration downregulates select BDNF transcript and protein levels in the dorsolateral prefrontal cortex of rhesus monkeys. Frontiers in Psychiatry, 14, 1054506.

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Measuring Cytokine and Chemokine Secretion

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Conditioned culture media were collected at day 3 post TNF-treatment, centrifuged for 10 min at 4 °C to remove cell debris and stored at −80 °C until use. Measurement of released cytokines and chemokines including CCL2(also known as MCP1), CCL3(MIP1α), CCL4(MIP1β), CCL5(RANTES), CCL7(MCP3), CXCL1,2,3(GRO), CXCL8(IL8), CXCL10(IP10), CXCL12(SDF1α+β), CSF2(GMCSF), CSF3(GCSF), FGF2, IL1RN(IL1ra), IL1B(IL1β), IL6, LIF were by MILLIPLEX^® Multiplex Assays with Luminex LX200 instrument (Millipore) according to the manufacturer’s instruction. Analysis was performed using MILLIPLEX^® Analyst Software Version 3.4 (Millipore).

Lee C., Low C.Y., Wong S.Y., Lai M.K, & Tan M.G. (2017). Selective induction of alternatively spliced FynT isoform by TNF facilitates persistent inflammatory responses in astrocytes. Scientific Reports, 7, 43651.

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Measuring Allergic Biomarkers in Serum and Urine

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Serum Dp- and Df-specific IgE levels were measured by fluorescent enzyme immunoassay using the ImmunoCAP^® system (Thermo Fisher Scientific, Uppsala, Sweden). IL-4, IL-5, IL-10, IL-13, and IFN-γ levels were measured in serum using a Human High Sensitivity T-Cell Magnetic Bead Panel kit (Millipore, Billerica, MA, USA) and Luminex LX200 (Millipore). Urinary LTE₄ and PGF_2α levels were measured using a commercial ELISA kit (Cayman Chemical, Ann Arbor, MI, USA) and expressed as ng/mmol creatinine. Creatinine level was measured using a CREJ2 kit with Cobas 8000 (Roche Diagnostics, Mannheim, Germany).

Kang M.G., Han S.W., Kang H.R., Hong S.J., Kim D.H, & Choi J.H. (2020). Probiotic NVP-1703 Alleviates Allergic Rhinitis by Inducing IL-10 Expression: A Four-week Clinical Trial. Nutrients, 12(5), 1427.

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