Thirty μg total protein melanoma lysates (MPER, Thermo Fisher – 78501) were separated on 4-15% Bis-Tris gels (Bio-Rad) and transferred to nitrocellulose membranes. The membranes were incubated overnight with antibody (Dilutions - 1:1000 for YAP/TAZ antibody (Cell Signaling Technology – D24E4), 1:1000 ARPC5 (Proteintech - 16717-1-AP), 1:1000 ARPC5L (Proteintech – 22025-1-AP), 1:10000 for GAPDH (Cell Signaling – D16H11)) in nonfat 5% milk (Santa Cruz – sc-2324). The membranes were washed with 1X TBS-T four times for 15 minutes and incubated with 1:4000 anti-rabbit IgG HRP-linked antibody (Cell Signaling – 7074). Membranes were developed with Clarity Western ECL substrate according to the manufacturer’s instructions (Bio-Rad). Blots were normalized with GAPDH or total protein input (Stain Free System, Bio-Rad). All Western analysis shown are representatives of at least three independent experiments.
Yap taz antibody
Manufactured by Cell Signaling Technology
The YAP/TAZ antibody is a reagent used in research applications to detect the expression of the YAP (Yes-associated protein) and TAZ (transcriptional co-activator with PDZ-binding motif) proteins. YAP and TAZ are key transcriptional regulators that play crucial roles in cellular processes such as proliferation, differentiation, and organ development.
Automatically generated - may contain errors
Lab products found in correlation
Clarity western ecl substrate, by Bio-Rad (2 mentions)
Bis tris gel, by Bio-Rad (1 mentions)
Stain free system, by Bio-Rad (1 mentions)
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
M per, by Thermo Fisher Scientific (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions)
2 protocols using yap taz antibody
1
Western Blot Analysis of Melanoma Proteins
2
Western Blot Analysis of YAP/TAZ Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed in RIPA buffer and 30 μg total protein was separated on 4-15% Bis-Tris gels and subsequently transferred to nitrocellulose membranes. The membranes were then probed overnight with 1:1000 YAP/TAZ antibody (Cell Signaling) and 1:10000 GAPDH (Cell Signaling). Membranes were washed with 1X TBS-T three times for 20 minutes and incubated with 1:4000 goat anti-rabbit IgG-HRP (Santa Cruz) and developed with Clarity Western ECL substrate according to the manufacturer's instructions (Bio-Rad).
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