Avance 3 400 nanobay

Manufactured by Bruker

The AVANCE III 400 Nanobay is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for analytical and research applications. It features a 400 MHz superconducting magnet and advanced hardware and software capabilities to enable efficient data acquisition and processing.

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Lab products found in correlation

Silica gel 60n, by Kanto Chemical (1 mentions) Dpx 400, by Bruker (1 mentions) Uv 4802 spectrophotometer, by Unico (1 mentions)

Spelling variants of this product

Avance III (981 citations) Avance 400 (545 citations) Avance III 400 (252 citations) Avance 400 Nanobay (6 citations) Avance III HD NanoBay 400 MHz (6 citations) Avance III Nanobay 400 MHz spectrometer (4 citations) Avance III Nanobay (2 citations) Avance III HD nanobay AX-400 (2 citations) NanoBay AVANCE III 400 MHz NMR spectrometer (2 citations) Avance III HD nanobay 400 (2 citations)

4 protocols using avance 3 400 nanobay

Synthesis of Benzil-PIC Compound

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All reactions were monitored by thin-layer chromatography carried out on 0.2 mm E. Merck silica gel plates (60F-254). Column chromatography was performed on silica gel (Silica Gel 60N (spherical, neutral), 40–50 μm, Kanto Chemical Co., Inc.). ¹H NMR spectra were recorded at 400 MHz on a Bruker AVANCE III 400 NanoBay. DMSO-d₆ and CDCl₃ were used as deuterated solvents. Mass spectra (ESI-TOF-MS) were measured by using a Bruker micrOTOFII-AGA1. All reagents were purchased from TCI, Wako Co. Ltd., Aldrich Chemical Company, Inc. and Kanto Chemical Co., Inc., and were used without further purification.
The synthetic procedure of Benzil-PIC is shown in Scheme 2. The synthetic procedure is analogous to that of PIC [24 (link)].

Kobayashi Y., Mamiya Y., Mutoh K., Sotome H., Koga M., Miyasaka H, & Abe J. (2019). Excited state dynamics for visible-light sensitization of a photochromic benzil-subsituted phenoxyl-imidazolyl radical. Beilstein Journal of Organic Chemistry, 15, 2369-2379.

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Characterization of Organic Compounds

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Melting points were carried out with a Köfler hot stage or Digital Electrothermal melting point apparatus. Nuclear magnetic resonance (¹H NMR and ¹³C NMR-APT) spectra were determined in CDCl₃ or DMSO-d₆ and were recorded with a Bruker Avance III 400 NanoBay. Chemical shifts are reported in parts per million (ppm) downfield from tetramethylsilane (TMS) used as the internal standard. Splitting patterns were designated as follows: s, singlet; d, doublet; t, triplet; q, quadruplet; quin, quintet; sext, sextet; sept, septet; m, multiplet; br s, broad singlet; dd, doublet of doublets. Mass spectra (MS) were performed on combined Liquid Chromatograph-Agilent 1100 series Mass Selective Detector (MSD). Analytical thin-layer chromatography (TLC) was performed on Merck silica gel F-254 plates. Pure compounds showed a single spot in TLC. For flash chromatography, Merck silica gel 60 was used with particle sizes 0.040 and 0.063 mm (230 and 400 mesh ASTM).

Ibba R., Corona P., Nonne F., Caria P., Serreli G., Palmas V., Riu F., Sestito S., Nieddu M., Loddo R., Sanna G., Piras S, & Carta A. (2023). Design, Synthesis, and Antiviral Activities of New Benzotriazole-Based Derivatives. Pharmaceuticals, 16(3), 429.

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Synthesis and Characterization of CoA-PEG Nile Red

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CoA-PEG Nile Red compounds were synthesized by the same procedures as previously (Sundukova et al., 2019 (link)). All chemical reagents and anhydrous solvents for synthesis were purchased from commercial suppliers (Sigma-Aldrich, Fluka, Acros) and were used without further purification or distillation. The composition of mixed solvents is given by the volume ratio (vol/vol). ¹H magnetic resonance (NMR) spectra were recorded on a Bruker DPX 400 (400 MHz) or Bruker AVANCE III 400 Nanobay (400 MHz) with chemical shifts (δ) reported in ppm relative to the solvent residual signals of CD₃OD (3.31 ppm for ¹H) or DMSO-d₆ (2.50 ppm for ¹H). Coupling constants are reported in Hz. High-resolution mass spectra (HRMS) were measured on a Micromass Q-TOF Ultima spectrometer with electrospray ionization (ESI) or LTQ Orbitrap ELITE ETD (Thermo Fisher Scientific). Preparative RP-HPLC was performed on a Dionex system equipped with an UVD 170U UV-Vis detector for product visualization on a Waters SunFire Prep C18 OBD 5 µm 10 × 150 mm Column (buffer A: 0.1% TFA in H₂O; buffer B: acetonitrile. Typical gradient was from 0 to 100% B within 30 min with 4 ml/min flow).

Umebayashi M., Takemoto S., Reymond L., Sundukova M., Hovius R., Bucci A., Heppenstall P.A., Yokota H., Johnsson K, & Riezman H. (2022). A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins. The Journal of Cell Biology, 222(3), e202206119.

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Spectroscopic Characterization of Organic Compounds

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All the reagents were supplied by commercial suppliers and were directly used without further purification. Absorption spectra were recorded on a UNICO UV-4802 spectrophotometer. Fluorescence spectra were obtained on a fluorescence spectrophotometer (Lengguang tech CO., Ltd. F97XP, China). ¹H NMR and ¹³C NMR spectra were recorded on a Bruker AVANCE III 400 Nanobay at 500 MHz for ¹H NMR and 300 MHz for ¹³C NMR (TMS as an internal standard). High-resolution mass spectra (HRMS) were recorded on a MicrOTOF Bruker. The pH values were measured with an acidity meter (alkalis, pH 400, China).

Deng Z., Li F., Zhao G., Yang W, & Hu Y. (2020). A mitochondrion-targeted dual-site fluorescent probe for the discriminative detection of SO32− and HSO3− in living HepG-2 cells. RSC Advances, 10(44), 26349-26357.

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