The analysis of ciprofloxacin was adopted from the United States Pharmacopoeia (USP) and was performed using HPLC (Shimadzu HPLC, model LC-2030C PLUS 3D). The system included an integrated solvent and degasser, an analytical pump, a thermostatic autosampler, a UV detector, and a thermostatic column compartment. Data acquisition was performed via the LabSolutions LCGC software. The eluent was detected at 278 nm. Separation was carried out using a reversed-phase Interclone C18 column (250 mm × 4.6 mm, 5 μm particle ODS 100 Å size) (Phenomenex, California, USA) at 30 °C. The mobile phase had an isocratic composition of 0.025 M phosphoric acid (pH 3.0 ± 0.1) previously adjusted with triethylamine/acetonitrile (80:20 v/v), eluted at a flow rate of 1.5 mL/min. The injection volume was 20 µL. The drug concentration was then determined according to a calibration curve. The calibration curve was plotted by correlating the peak area measured with known concentrations of ciprofloxacin samples. All measurements were conducted in triplicate to calculate mean values and standard deviations.
Lc 2030c 3d plus
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu LC-2030C 3D Plus is a high-performance liquid chromatography (HPLC) system. It features a compact design and offers a comprehensive range of modules for various analytical applications. The LC-2030C 3D Plus provides precise and reliable separation, detection, and quantification of a wide variety of chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Inertsustain c18, by GL Sciences (1 mentions)
Sb aq, by Agilent Technologies (1 mentions)
Gemcitabine, by Selleck Chemicals (1 mentions)
400 mhz instrument, by JEOL (1 mentions)
Q exactive plus, by Thermo Fisher Scientific (1 mentions)
Lcms 2020, by Shimadzu (1 mentions)
Prominence i, by Shimadzu (1 mentions)
Sephadex lh 20 column, by GE Healthcare (1 mentions)
Waters 2695, by Waters Corporation (1 mentions)
Avance 500 mhz spectrometer, by Bruker (1 mentions)
12 protocols using lc 2030c 3d plus
1
HPLC Analysis of Ciprofloxacin in Pharmacopoeia
2
HPLC Quantification of Paracetamol
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Paracetamol content in each trial was determined using HPLC. HPLC, Prominence, LC-2030C plus 3D, Shimadzu (Japan). Nucleodur (MN)150 mm × 3.9 mm (5 µm) at 290 nm was used for HPLC assay. The mobile phase consisted of acetonitrile/phosphate buffer (pH 3) (10:90, v/v). The flow rate was 1.5 ml/min and the retention time of paracetamol was 2.061 min. The injection volume was 20 µl.
3
Analytical Procedure for Sample Analysis
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The equipment used in this study has been calibrated according to ISO/IEC 17025 and GLP which include a liquid chromatography system of Shimadzu -LC-2030C 3D Plus with DAD (Shimadzu, Japan), a solid-phase extraction (SPE) system (Supelco, US), an analytical balance, with the accuracy of 0.01 mg (Metler Toledo), and other common laboratory equipment. The chromatography column was InertSustain C18 (250 × 4.6 mm; 5 µm) (GL Sciences, Japan) and the C18 SPE column (6 mL, 500 mg) (SilactSPE TM Affinisep, France).
4
Organic Acid Quantification by HPLC
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Organic acids were determined by LC-2030C 3DPLus (Shimadzu, Japan) equipped with a C18 column (ZORBAX SB-Aq, 4.6 mmｘ250mm, 5 μM particle size) and a UV detector. The measurements were acquired according to the method described by Huang et al. (2017) (link) with minor modifications. The mobile phase was 2% methanol and 98% 20 mmol/L NaH2PO4 (pH 2.7, adjusted with H3PO4), and the column temperature was set at 35 °C with a flow rate of 0.4 mL/min, injection volume 10 μL, and the wavelength of UV detector was set as 210 nm.
5
ARCC-4 Solubility in Intestinal pH
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The saturation solubility was determined in 0.05 M phosphate buffer (pH 6.8 medium, simulating the intestinal pH) by using the shaking flask method. ARCC-4 was added in excess, and flasks were shaken at 37 °C for 48 h. Before analysis, samples were centrifugated for 5 min at 21,000× g, and 37 °C. Then, 450 µL of the supernatant was diluted with 50 µL acetonitrile to prevent precipitation. The solubility was quantified by high-performance liquid chromatography (HPLC) (Shimadzu LC-2030C 3D Plus) using a reversed-phase C18 column and a diode array detector. A volume of 100 µL was injected into a mobile phase containing 80% acetonitrile and 20% demineralized water. The measurement was performed at 268 nm.
6
Synthesis and Cytotoxicity Analysis of Novel Gemcitabine Derivatives
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All the chemicals and reagents
were purchased from Sigma-Aldrich, SRL, and Sd fine and were used
without further purification. The hLDHA enzyme was
purchased from Sigma-Aldrich. Pancreatic cancer cells (02.03, 04.03,
03.27) and liver cancer cells (HepG2) were purchased from ATCC and
NCCS, respectively. Gemcitabine was procured from Selleckchem. Stock
solution of 1 mM was prepared in DMSO and used for in vitro cytotoxicity analysis in pancreatic cancer cells. Thin-layer chromatography
(TLC) was performed on silica gel 60 F254 Merck KGaA Germany, and
spots were visualized by iodine vapor or by irradiation with ultraviolet
light (254 nm). Silica gel of 100–200 mesh was obtained for
column chromatography. Melting points (mp) of all7a–d,
7j, 7l, and 7m and 8a–d, 8j, 8l, and 8m were calculated on a JSGW apparatus and are
uncorrected. Solvent DMSO was used for NMR purchased from Sigma. NMR
spectra were recorded on a Bruker WH-400 spectrometer or JEOL 400
MHz instrument at a ca. 5–15% (w/v) solution
in DMSO-d6. Mass spectra were recorded
on a Q EXACTIVE PLUS, Thermo Scientific spectrometer. Elemental analysis
was carried out on a Vario ELIII elementor. HPLC analysis was performed
on Shimadzu LC-2030C 3D plus using column XTIMATE C18 and flow rate
1.0 mL/min.
were purchased from Sigma-Aldrich, SRL, and Sd fine and were used
without further purification. The hLDHA enzyme was
purchased from Sigma-Aldrich. Pancreatic cancer cells (02.03, 04.03,
03.27) and liver cancer cells (HepG2) were purchased from ATCC and
NCCS, respectively. Gemcitabine was procured from Selleckchem. Stock
solution of 1 mM was prepared in DMSO and used for in vitro cytotoxicity analysis in pancreatic cancer cells. Thin-layer chromatography
(TLC) was performed on silica gel 60 F254 Merck KGaA Germany, and
spots were visualized by iodine vapor or by irradiation with ultraviolet
light (254 nm). Silica gel of 100–200 mesh was obtained for
column chromatography. Melting points (mp) of all
7j, 7l,
uncorrected. Solvent DMSO was used for NMR purchased from Sigma. NMR
spectra were recorded on a Bruker WH-400 spectrometer or JEOL 400
MHz instrument at a ca. 5–15% (w/v) solution
in DMSO-d6. Mass spectra were recorded
on a Q EXACTIVE PLUS, Thermo Scientific spectrometer. Elemental analysis
was carried out on a Vario ELIII elementor. HPLC analysis was performed
on Shimadzu LC-2030C 3D plus using column XTIMATE C18 and flow rate
1.0 mL/min.
7
HPLC Analysis of Licorice Phytochemicals
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HPLC analysis was carried out as described by Wei, Yang, Chen, Wang, & Cui, (2015) .
A LC-2030C 3D Plus (Shimadzu) device equipped with a quaternary pump, autoinjector and DAD detector was used. The column was ACE Kromasil 100 C18 (250 x 4.6 mm; 5 μm) and analyses were accomplished at 298 K. The mobile phase comprised acetonitrile (A) and 0.026% aqueous H 3 PO 4 (v/v), and the following elution gradient was applied: 20-25% A for 0-20 min, 25-34% A for 20-30 min, 34-50% A for 30-50 min, 50-60% A at 50-60 min and 60% A for 60-80 min. The initial conditions were attained in 5 min. The flow rate was 0.7 ml/min and was kept constant throughout the analysis. The injection volume was 20 μl and the detections were carried out at 230, 254, 280 and 370 nm. Calibration curves with standards were used to determine the content of the bioactive licorice phytochemicals (liquiritin, liquiritigenin, isoliquiritigenin, glabridin and glycyrrhizic acid) in the different samples.
A LC-2030C 3D Plus (Shimadzu) device equipped with a quaternary pump, autoinjector and DAD detector was used. The column was ACE Kromasil 100 C18 (250 x 4.6 mm; 5 μm) and analyses were accomplished at 298 K. The mobile phase comprised acetonitrile (A) and 0.026% aqueous H 3 PO 4 (v/v), and the following elution gradient was applied: 20-25% A for 0-20 min, 25-34% A for 20-30 min, 34-50% A for 30-50 min, 50-60% A at 50-60 min and 60% A for 60-80 min. The initial conditions were attained in 5 min. The flow rate was 0.7 ml/min and was kept constant throughout the analysis. The injection volume was 20 μl and the detections were carried out at 230, 254, 280 and 370 nm. Calibration curves with standards were used to determine the content of the bioactive licorice phytochemicals (liquiritin, liquiritigenin, isoliquiritigenin, glabridin and glycyrrhizic acid) in the different samples.
8
HPLC Analysis of Licorice Phytochemicals
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HPLC analysis was carried out as described by Wei, Yang, Chen, Wang, & Cui, (2015) .
A LC-2030C 3D Plus (Shimadzu) device equipped with a quaternary pump, autoinjector and DAD detector was used. The column was ACE Kromasil 100 C18 (250 x 4.6 mm; 5 μm) and analyses were accomplished at 298 K. The mobile phase comprised acetonitrile (A) and 0.026% aqueous H 3 PO 4 (v/v), and the following elution gradient was applied: 20-25% A for 0-20 min, 25-34% A for 20-30 min, 34-50% A for 30-50 min, 50-60% A at 50-60 min and 60% A for 60-80 min. The initial conditions were attained in 5 min. The flow rate was 0.7 ml/min and was kept constant throughout the analysis. The injection volume was 20 μl and the detections were carried out at 230, 254, 280 and 370 nm. Calibration curves with standards were used to determine the content of the bioactive licorice phytochemicals (liquiritin, liquiritigenin, isoliquiritigenin, glabridin and glycyrrhizic acid) in the different samples.
A LC-2030C 3D Plus (Shimadzu) device equipped with a quaternary pump, autoinjector and DAD detector was used. The column was ACE Kromasil 100 C18 (250 x 4.6 mm; 5 μm) and analyses were accomplished at 298 K. The mobile phase comprised acetonitrile (A) and 0.026% aqueous H 3 PO 4 (v/v), and the following elution gradient was applied: 20-25% A for 0-20 min, 25-34% A for 20-30 min, 34-50% A for 30-50 min, 50-60% A at 50-60 min and 60% A for 60-80 min. The initial conditions were attained in 5 min. The flow rate was 0.7 ml/min and was kept constant throughout the analysis. The injection volume was 20 μl and the detections were carried out at 230, 254, 280 and 370 nm. Calibration curves with standards were used to determine the content of the bioactive licorice phytochemicals (liquiritin, liquiritigenin, isoliquiritigenin, glabridin and glycyrrhizic acid) in the different samples.
9
LCMS-Based Compound Identification Protocol
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Confirmation of the identity of the components isolated by preparative HPLC was carried out using a prominence-I (LC-2030C 3D Plus) and LCMS-2020 (Shimadzu, Japan). Samples (20 μL) were analyzed for scanning mode. The mobile phase was 50% methanol in water, and the flow rate was 0.1 mLmin−1. MS interface was operated in positive ion mode using a cone voltage of 20 V, capillary voltage of 3.0 kV, and collision energy of 12 eV. The ion source temperature was maintained at 140 °C and the dissolution gas temperature at 340 °C. The flow rates of nitrogen (Peak Scientific) and cone gases were 460 Lh−1 and 67 Lh−1 respectively.
10
Lignin Molecular Weight Distribution Analysis
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The molecular weight distribution of the [N11H(2OH)][LAC]-extracted lignin was analyzed using high-performance liquid chromatography (HPLC) (Shimadzu Prominence-i, LC-2030C 3D Plus, Shimadzu Corporation, Kyoto, Japan) equipped with LabSolutions GPC software. The HPLC system contained a pump, an auto-sampler, a set of 2 MCX columns (1000 Å and 100,000 Å), and a pre-column (8 mm × 50 mm) (Polymer Standards Service (PSS), GmbH, Mainz, Germany) with a UV detector (280 nm). The lignin samples were dissolved in 0.1 M NaOH (5 mg/mL), and isocratic flow was maintained with 0.1 M NaOH solution at a flow rate of 0.5 mL/min with an injection volume of 20 µL. The relative molecular weight of the lignin was determined using polystyrene sulfonate sodium salt standards (PSS, GmbH, Mainz, Germany) ranging from 1100 Da to 100,000 Da.
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