Treated cells were lysed in RIPA lysis buffer (Beyotime, Shanghai, China). An equal amount of protein of about 20 μg was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto the polyvinylidene fluoride membrane. After blocking with 5% skimmed milk, the polyvinylidene fluoride membrane was incubated with anti-ARAP3 antibody (Abcam, USA). After washing three times with tris-buffered saline and Tween 20, the membrane was incubated with horseradish peroxidase-linked secondary anti-goat immunoglobulin G antibody (Abcam) at room temperature for 1.5 h. GAPDH protein, detected using an anti-GAPDH antibody (Abcam), was used for control.
Horseradish peroxidase linked secondary anti goat immunoglobulin g antibody
Manufactured by Abcam
Horseradish peroxidase-linked secondary anti-goat immunoglobulin G antibody is a laboratory reagent used for detecting and quantifying goat immunoglobulin G proteins in samples. The antibody is conjugated with the enzyme horseradish peroxidase, which can be used to catalyze a colorimetric or chemiluminescent reaction for signal detection.
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Lab products found in correlation
2 protocols using horseradish peroxidase linked secondary anti goat immunoglobulin g antibody
1
Western Blot Analysis of ARAP3
2
Protein Extraction and Western Blot Analysis
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The total cellular protein was extracted using a RIPA protein lysis buffer (Beyotime, Shanghai, China). Equal amounts of protein (20 µg) were loaded and separated by SDS-PAGE and transferred onto the polyvinylidene fluoride membrane. After blocking with 5% skimmed milk, the membrane was incubated with a relative antibody (Abcam, Cambridge, UK) overnight at 4°C. Then, the membrane was washed and incubated with horseradish peroxidase-linked secondary anti-goat immunoglobulin G antibody (Abcam) at room temperature for 1 hour. GAPDH was used as internal control. All experiments were performed at least three times.
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