1640 media

Manufactured by Merck Group

Sourced in United States, Germany

1640 media is a cell culture media developed by Merck Group. It is a formulation designed to support the growth and maintenance of various cell types in in vitro experiments. The media provides the necessary nutrients and components to facilitate cell proliferation and survival. This product is intended for laboratory use and research purposes.

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RPMI-1640 media (314 citations) RPMI 1640 culture media (15 citations) RPMI 1640 cell culture media (8 citations) RPMI-1640 growth media (4 citations) RMPI 1640 media (2 citations) RPM1-1640 media (2 citations) Advance RPMI 1640 media (2 citations) Complete RPMI 1640 media (2 citations) RPMI-1640 modified media (2 citations) RPMI– 1640 phenol red-free media (2 citations)

5 protocols using 1640 media

Visualizing FITC-ROPPIP Uptake in HTR-8/Svneo Cells

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Human trophoblast cells (HTR-8/Svneo) were acquired from Wuhan Procell Life Technology Co., Ltd. (catalog number: CL-0599, Wuhan, China). The cells were cultured with Roswell Park Memorial Institute (RPMI) 1640 media (Sigma, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 mg/mL streptomycin double-antibody, which was placed in a constant temperature and humidity incubator containing 5% carbon dioxide (CO₂) at 37 °C. The culture media was changed every day and the cells were passaged when they reached 90% confluence. HTR-8/Svneo cells were administrated with 100 µM FITC-ROPPIP and incubated for 1 h at 37 °C in the dark, then imaged with a Confocal laser scanning microscopy (magnification, ×200).

Ling L., Yuan X., Liu X., Pei W, & Li R. (2021). A novel peptide promotes human trophoblast proliferation and migration through PI3K/Akt/mTOR signaling pathway. Annals of Translational Medicine, 9(12), 981.

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Gold(III) Chloride-Based Cancer Therapy

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Gold(III) chloride trihydrate (99.9% trace metals basis), phosphate buffer saline (PBS, pH 7.4), sodium citrate dihydrate, doxorubicin hydrochloride, fetal bovine serum, Roswell Park Memorial Institute (RPMI) 1640 media, and Fluoroshield^TM with 4′,6-diamidino-2-phenylindole (DAPI, histology mounting medium) were purchased from Sigma-Aldrich. Thiol terminated binding DNA (5′-HS-CC-AAAAAAAAAA-TCG-TCG-TCG-TCG-TCG-TCG-TCG-3′) and capture DNA (5′-CGA-CGA-CGA-CGA-CGA-CGA-CGA-3′) were synthesized by GenoTech Co., (Daejeon, Korea).

Lee C.S., Kim T.W., Oh D.E., Bae S.O., Ryu J., Kong H., Jeon H., Seo H.K., Jeon S, & Kim T.H. (2020). In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment. Cancers, 12(3), 634.

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Establishing Docetaxel-Resistant CRPC Cell Lines

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PCa cell lines were obtained from the Chinese Academy of Science Cell Bank (Shanghai, China). The human CRPC cell lines PC-3 and DU145 were cultured in Roswell Park Memorial Institute (RPMI) 1640 media (Sigma, Darmstadt, Germany; Catalog No. R8758) containing 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA, Catalog No. 10091). Cell cultures were maintained at 37 ℃ in a humidified atmosphere with 5% CO₂ and 95% air. Docetaxel and cabazitaxel were acquired for the study (SelleckChem, Houston, TX, USA, Catalog No. S1148 and S3022, respectively). The CRPC cells were exposed to Docetaxel or cabazitaxel at a dose of 2 nmol/L for 24 h. Furthermore, both PC-3 and DU145 cells were treated with Docetaxel (2 nmol/L) for 2 weeks to induce resistance. Upon observing that Docetaxel did not affect cell growth, we concluded that these cells were resistant to Docetaxel (Additional file 2).

Li H., Wang X., Zhai M., Xu C, & Chen X. (2024). Exploration of the influence of GOLGA8B on prostate cancer progression and the resistance of castration-resistant prostate cancer to cabazitaxel. Discover Oncology, 15, 152.

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FGFR-1c Activation Quantification in BaF3 Cells

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BaF3 murine lymphoid cells were transfected to express the single FGFR-1c isoform so that specific FGFR-1c activation can be quantified.^{38 (link)} Cells were maintained in Roswell Park Memorial Institute 1640 media (Sigma Chemical, St. Louis, MO) supplemented with 10% newborn bovine serum (Sigma Chemical), 50-μM β-mercaptoethanol, 0.5 ng/mL murine recombinant interleukin-3 (PeproTech Inc, Rocky Hill, NJ), 2-mM L-glutamine, penicillin-streptomycin (“BaF3 culture medium”), and G418 antibiotic (600 μg/mL). FGFR-1c expressing BaF3 cells were washed twice in BaF3 “assay media” (“culture media” lacking both murine recombinant interleukin-3 and β-mercaptoethanol) and plated at a density of 30,000 cells per well in a 96-well assay plate in assay media containing heparin sulfate (1 μg/mL) and concentrations of recombinant WT FGF-1 and Cys-free mutants ranging from 0.02 to 5 nM (3.18 × 10²–7.95 × 10⁵ pg/mL). The cells were incubated for 36 h, and DNA synthetic activity was determined by adding 1 μCi of ³H-thymidine in 50 μL of BaF3 assay medium to each well. Cells were harvested after 4 h by filtration through glass fiber paper. Incorporated ³H-thymidine was counted on a MicroBeta plate scintillation counter (PerkinElmer, Waltham, MA).

Xia X., Kumru O.S., Blaber S.I., Middaugh C.R., Li L., Ornitz D.M., Sutherland M.A., Tenorio C.A, & Blaber M. (2016). Engineering a Cysteine-Free Form of Human Fibroblast Growth Factor-1 for “Second Generation” Therapeutic Application. Journal of pharmaceutical sciences, 105(4), 1444-1453.

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Culturing and Priming Cell Lines for Experimentation

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The murine macrophage-like cell line RAW 264.7, human cervical adenocarcinoma cell line HeLa, human colorectal adenocarcinoma cell line Caco-2 were maintained in Dulbecco's Modified Eagle's Media (Sigma-Aldrich) supplemented with 10% FCS (Fetal calf serum, Gibco) at 37 0 C temperature in the presence of 5% CO2. Human monocyte cell line U937 cells were maintained in Roswell Park Memorial Institute 1640 media (Sigma-Aldrich) supplemented with 10% FCS (Fetal calf serum, Gibco). For polarizing the Caco-2 cells, DMEM media was further supplemented with 1% non-essential amino acid solution (Sigma-Aldrich). Phorbol Myristate Acetate (Sigma-Aldrich) (concentration-20 ng/ mL) was used for the activation of U937 cells for 24 hours at 37 0 C temperature in the presence of 5% CO2, followed by the replacement of the media carrying PMA with normal RPMI supplemented with 10% FCS and further incubating the cells for 24 hours before starting the experiments.

Chowdhury A.R., Sah S., Varshney U., & Chakravortty D. (2021). Salmonella Typhimurium outer membrane protein A (OmpA) renders protection against nitrosative stress by promoting SCV stability in murine macrophages.

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