Mouse hepatocytes were isolated using either collagenase type I digestion (Sigma-Aldrich, St Louis, Mo) or enzymes from the mouse liver dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Details of both protocols are provided in in the Methods section in the Online Repository .
Collagenase type 1 digestion
Manufactured by Merck Group
Sourced in Sao Tome and Principe, United States
Collagenase type I digestion is a laboratory enzyme used for the dissociation and isolation of cells from various tissue types, particularly connective tissues. It functions by breaking down the collagen present in the extracellular matrix.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using collagenase type 1 digestion
1
Mouse Hepatocyte Isolation Protocols
2
Isolation and Culture of PDLSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tissues of the periodontal ligaments were minced into small pieces and treated with collagenase type I digestion (1 mg/mL; Sigma-Aldrich, NJ, USA) at 37°C for 0.25 h. The cell suspensions were then filtered and cultured in DMEM (Gibco, California, USA) containing 10% FBS (Gibco, California, USA), 2 mM L-glutamine (Gibco, California, USA), and 1% penicillin/streptomycin (Gibco, California, USA) [24 (link)]. Cells were digested after 14 days of incubation and single-cell-derived colony cultures were obtained using the limiting dilution technique. PDLSCs were obtained by dilution and passed five times.
The PDLSCs were divided into two groups, the control and LPS-PG groups. The final concentration of 1 μg/mL LPS-PG was added to the LPS-PG group [25–27 (link)]. Follow-up experiments were conducted after LPS-PG treatment 24 h later.
The PDLSCs were divided into two groups, the control and LPS-PG groups. The final concentration of 1 μg/mL LPS-PG was added to the LPS-PG group [25–27 (link)]. Follow-up experiments were conducted after LPS-PG treatment 24 h later.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!