Protein extraction, two-dimensional polyacrylamide gel electrophoresis, and transfer to nitrocellulose membrane were carried out as previously described [11 (link),12 (link),15 (link)]. The membrane was incubated with the following antibodies: cleaved CASP3, cleaved PARP, PARP, p-EGFR, EGFR, p-AKT, AKT, p-ERK, ERK, p-STAT3, STAT3, eIF4A3, p-4E-BP1, mTOR, p-P70-S6, MUC1, and MUC1-C, purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody to glyceraldehyde-3-phosphate dehydrogenase was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Antibody to glyceraldehyde 3 phosphate dehydrogenase
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Antibody to glyceraldehyde-3-phosphate dehydrogenase is a laboratory reagent used to detect the presence and quantity of the GAPDH protein in biological samples. GAPDH is a key enzyme involved in glycolysis, the metabolic process that converts glucose to energy. This antibody can be used in various techniques, such as Western blotting, immunohistochemistry, and ELISA, to identify and quantify GAPDH in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
P erk, by Cell Signaling Technology (2 mentions)
P egfr, by Cell Signaling Technology (2 mentions)
Cleaved parp, by Cell Signaling Technology (2 mentions)
P stat3, by Cell Signaling Technology (2 mentions)
P akt, by Cell Signaling Technology (2 mentions)
Cleaved casp3, by Cell Signaling Technology (2 mentions)
Stat3, by Cell Signaling Technology (2 mentions)
P p70s6, by Cell Signaling Technology (2 mentions)
Muc1 c, by Cell Signaling Technology (2 mentions)
Eif4a3, by Cell Signaling Technology (2 mentions)
P 4ebp1, by Cell Signaling Technology (2 mentions)
2 protocols using antibody to glyceraldehyde 3 phosphate dehydrogenase
1
Protein Expression Analysis by Western Blot
2
Protein Expression Analysis in Cancer Cells
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Protein extraction, two-dimensional polyacrylamide gel electrophoresis, and transfer to nitrocellulose membrane were carried out as previously described 11, (link)12, (link)15 (link) . The membrane was incubated with the following antibodies: cleaved CASP3, cleaved PARP, PARP, p-EGFR, EGFR, p-AKT, AKT, p-ERK, ERK, p-STAT3, STAT3, eIF4A3, p-4E-BP1, mTOR, p-P70-S6, MUC1, and MUC1-C purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody to glyceraldehyde-3-phosphate dehydrogenase was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
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