Protein extracts were done from renal cortical tissues by a standard method, and a total of 40 μg of protein was electrophoresed on 10% SDS-PAGE minigels, and the membranes were incubated in blocking buffer overnight at 4 °C with rabbit polyclonal anti-TGFβ1 antibodies (1:200; Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-type I collagen α1 antibodies (1:2000, Abcam Plc, Boston, MA, USA), goat polyclonal anti-toll-like receptor 4 (TLR4) antibody (1:500; Santa Cruz Biotechnology), rabbit polyclonal anti-GLP1 receptor antibody (1:100, Abcam Plc), and mouse monoclonal anti-β-actin antibodies (1:5000, Sigma-Aldrich Corp, Saint Louis, MO, USA). The membranes were subsequently incubated with horseradish peroxidase-conjugated secondary antibodies (1:1000 dilution) for 60 min at room temperature. Immunoreactive bands were detected using enhanced chemiluminescence reagents (Amersham, Buckinghamshire, UK).
Goat polyclonal anti type 1 collagen α1 antibodies
Manufactured by Abcam
Sourced in United States
Goat polyclonal anti-type I collagen α1 antibodies are laboratory reagents designed for the detection and analysis of type I collagen α1. They are produced in goats and exhibit polyclonal reactivity.
Automatically generated - may contain errors
2 protocols using goat polyclonal anti type 1 collagen α1 antibodies
1
Western Blot Analysis of Renal Proteins
2
Protein Expression Profiling in Renal Tissue
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Protein extracts were prepared from renal cortical tissue samples and cells using a standard method, and 40 μg of protein was electrophoresed on 10% SDS-PAGE minigels, and the membranes were incubated in blocking buffer overnight at 4 °C with rabbit polyclonal anti-ATP-binding cassette transporter-1 antibodies (1:200, Abcam Plc), rabbit polyclonal anti-TGF-β1 antibodies (1:200; Santa Cruz Biotechnology), goat polyclonal anti-nephrin antibodies (1:500, Santa Cruz Biotechnology), goat polyclonal anti-type I collagen α1 antibodies (1:2000, Abcam Plc), goat polyclonal anti-3-hydroxy-3-methylglutaryl-coenzyme A (anti-HMG-CoA) reductase antibodies (1:100, Santa Cruz Biotechnology), rabbit polyclonal anti-osteopontin antibodies (1:1000, Abcam Plc), goat polyclonal anti-E-cadherin antibodies (1:500, Abcam Plc), rabbit polyclonal anti-FSP-1 antibodies (1:200, Abcam Plc) and mouse monoclonal anti-β-actin antibodies (1:5000, Sigma-Aldrich). The membranes were subsequently incubated with horseradish peroxidase-conjugated secondary antibodies (1:1000 dilution) for 60 min at room temperature. Immunoreactive bands were detected using enhanced chemiluminescence reagents (Amersham, Buckinghamshire, UK).
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