Toledo five easy plus ph meter

All chemicals and solvents were purchased from commercial sources and used without further purification. Solvents were purified by standard procedures. The water was purified by a Millipore filtration system. All chemical reactions were detected by thin-layer chromatography under 254 nm (or 365 nm) UV lamp. ¹H and ¹³C NMR spectra were recorded on a WIPM 400 spectrometer using tetramethylsilane (TMS) as the internal standard. High-resolution mass (HRMS) spectra were recorded on a Waters LCT Premier XE spectrometer using standard conditions (ESI, 70 eV). All absorption spectrums were measured on a UV-1800 UV visible spectrophotometer. All fluorescence spectra were measured on an FL-4500 fluorescence spectrometer. The pH measurements were taken on a METTLER TOLEDO FiveEasy Plus pH meter. The cell imaging experiments were taken under an Olympus IX71 inverted fluorescence microscope.