Reagents used were analytical grade and were obtained from the following sources: quercetin, FeSO4, CuSO4, and ZnCl2 were from MilliporeSigma (St. Louis, MO, USA); kaempferol was from Indofine (Hillsborough, NJ, USA), UDP-glucose was from Calbiochem (Gibbstown, NJ, USA), UDP- [U-14C] glucose was from PerkinElmer (Boston, MA, USA); Midiprep plasmid extraction kit was obtained from Promega (Madison, WI, USA). Miniprep plasmid extraction kit, MgCl2, MnCl2, KCl, and NaCl were purchased from Fisher Scientific (Waltham, MA, USA); CaCl2 was from Acros Organics (Morris Plains, Morris, NJ, USA); Na2SO4 was from Merk (Kenilworth, NJ, USA). Bovine thrombin was obtained from MP Biomedicals (Solon, OH, USA).
Bovine thrombin
Manufactured by MP Biomedicals
Sourced in United States, China
Bovine thrombin is a coagulation factor derived from bovine plasma. It plays a critical role in the blood clotting process by converting fibrinogen into fibrin, which is the key structural component of blood clots.
Automatically generated - may contain errors
Lab products found in correlation
Bovine fibrinogen, by MP Biomedicals (2 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Na2so4, by Merck Group (1 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
Cacl2, by Thermo Fisher Scientific (1 mentions)
Udp u 14c glucose, by PerkinElmer (1 mentions)
Mncl2, by Thermo Fisher Scientific (1 mentions)
Mgcl2, by Thermo Fisher Scientific (1 mentions)
Quercetin, by Merck Group (1 mentions)
Feso4, by Merck Group (1 mentions)
Cuso4, by Merck Group (1 mentions)
Zncl2, by Merck Group (1 mentions)
Porcine gelatin type a, by Merck Group (1 mentions)
Calcium chloride, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Yuanye Bio-Technology (1 mentions)
Urokinase, by Maclin Biochemical Technology (1 mentions)
Nanodiamond, by XFNANO (1 mentions)
5 protocols using bovine thrombin
1
Enzymatic Synthesis and Radiolabeling of Glycosides
2
Gelatin-Fibrin Matrix for Kidney Organoids
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A gelatin–fibrin matrix is produced using a previously reported method (26 (link)). Briefly, it is composed of 2 wt% (w/v) porcine gelatin type A (300 Bloom, Sigma), 10 mg/mL bovine fibrinogen (Millipore Sigma), and 2.5 mM Calcium chloride (Sigma), and cross-linked via a dual enzymatic cross-linking system that contains 0.2 wt% (w/v) transglutaminase (MooGloo, The Modernist Pantry), and 2 U/mL bovine thrombin (MP Biomedicals). Stock solutions of gelatin, fibrinogen, Calcium chloride, and transglutaminase are prepared in the indicated concentrations in PBS without calcium and magnesium and incubated for 15 min at 37 °C. After this pre-cross-linking step, 1 mL of this stock solution is mixed with thrombin and quickly cast into the chip device until the ECM covers the entire glass surface inside the device or into a 35-mm Petri dish for static controls. The ECM is incubated for 3 h at 37 °C before seeding kidney organoids onto the surface of it.
3
Fabrication of Fibrin Test Strips
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For fibrin test strip fabrication, 40 mg/mL bovine fibrinogen (MP Biomedicals) was combined with 10 U/mL bovine thrombin (MP Biomedicals) at a final concentration of 36 mg/mL and 1 U/mL, respectively, to catalyze the conversion of fibrinogen to fibrin67 (link). The fibrin mixture was casted into 3D printed plastic dog bone molds containing small Velcro pieces (VELCRO® Super-Grip Double-Head Hook) inserted at both ends to grip the gel during tensile testing. Molds were sprayed with Teflon (WD-40® SpecialistTM Dirt & Dust Resistant Dry Lube Spray) to facilitate mold release following casting. After 30 min at room temperature, the fibrin hydrogel solidified.
4
Nanodiamond-based Thrombus Dissolution
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Nanodiamond with the diameter of 5–10 nm (XFNANO Materials Tech Co., Ltd, China), melamine (Shanghai Aladdin Biochemical Technology Co., Ltd, China) and nitrogen gas (Shanghai Yangtai Chemical Technology Co., Ltd, China) were used as the raw material of N-AND. Phosphate buffered saline (PBS) was purchased from Yuanye Bio-Technology Co., Ltd, China. The calcium chloride solution (Yuanye Bio-Technology Co., Ltd, China), bovine whole blood (Beibo Biology Inc., China) and bovine thrombin (MP Biomedicals Co., Ltd, USA) were used to prepare the thrombus. Urokinase (Macklin Biochemical Co., Ltd, China) was initiated by bovine plasma (Yuanye Bio-Technology Co., Ltd, China) [44] (link).
5
Fabrication of Fibrin Test Strips
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For fibrin test strip fabrication, 40 mg/mL bovine fibrinogen (MP Biomedicals) was combined with 10 U/mL bovine thrombin (MP Biomedicals) at a final concentration of 36 mg/mL and 1 U/mL, respectively, to catalyze the conversion of fibrinogen to fibrin 52 (link) . The fibrin mixture was casted into 3D printed plastic dog bone molds containing small Velcro pieces (VELCRO® Super-Grip Double-Head Hook) inserted at both ends to grip the gel during tensile testing. Molds were sprayed with Teflon (WD-40® SpecialistTM Dirt & Dust Resistant Dry Lube Spray) to facilitate mold release following casting. After 30 minutes at room temperature, the fibrin hydrogel solidified.
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