According to the ‘National Clinical Laboratory Procedures’, routine testing methods were utilized. Drug susceptibility test was performed by paper diffusion method (K-B method). CLSI 2008–2010 operation procedures and result evaluation standards were used. Columbia blood plate, MH plates, identifying culture medium and drug sensitive slips were provided by the Oxoid (London, UK). The quality control strains viz. Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 were provided by provincial CDC clinical laboratory test center. The KB method is mainly used for the detection of ampicillin, cefotaxime, cefepime, ceftazidime, imipenem, clindamycin, vancomycin, erythromycin, roxithromycin, gentamicin and amikacin. The corresponding biological reagents were procured from Hangzhou Tianhe Microoragnism Reagents Co., Ltd. (Hangzhou, China). MH agar plates were stored at 4°C before use. Technicians with >5 years laboratory experience had performed all operations in strict accordance with the instructions.
Mh plates
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
MH plates are a type of microbiological growth medium used for the cultivation and identification of bacteria. They are designed to support the growth of a wide range of microorganisms. The plates provide a standardized and consistent substrate for culturing bacteria samples.
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Lab products found in correlation
2 protocols using mh plates
1
Antibiotic Susceptibility Testing for Bacterial Pathogens
2
Antibiotic Resistance Profiling of mcr-1 E. coli
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The antibiotic resistance profiles of the mcr-1-positive E. coli were determined by the disk diffusion assay [52 ]. The turbidity of bacterial cultures was standardized using a 0.5 McFarland standard and a spectrophotometer. The bacterial suspensions were then spread on Mueller-Hinton agar (MH) plates (Oxoid, Hampshire, England). Eighteen commercially available antibiotic discs, including penicillin (6 µg), ampicillin (10 µg), amoxicillin/clavulanic acid (20 µg/10 µg), cefixime (5 µg), cephalexin (30 µg), cefotaxime (30 µg), cefepime (30 µg), doripenem (10 µg), meropenem (10 µg), imipenem (10 µg), gentamicin (10 µg), kanamycin (30 µg), streptomycin (10 µg), ciprofloxacin (5 µg), norfloxacin (10 µg), tetracycline (30 µg), chloramphenicol (30 µg), and trimethoprim/sulfamethoxazole (25 µg) were added to the MH plates, which were incubated at 37 °C for 18–24 h. Erythromycin (15 µg) was used as a control, because E. coli is intrinsically resistant to this antibiotic [53 (link)]. Furthermore, E. coli DH5α was used for quality control. Antimicrobial susceptibility was interpreted by measuring the diameter of the zone of inhibition and comparing it to the Clinical and Laboratory Standards Institute (CLSI) [52 ] and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) standards [54 ].
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