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Goat f ab 2 anti human iga g m

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Goat F(ab')2 anti-human IgA/G/M is a secondary antibody product generated from goat serum. It recognizes and binds to the Fab regions of human IgA, IgG, and IgM antibodies. This product is commonly used in various immunological assays and techniques.

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Lab products found in correlation

Cytofix and phosflow perm wash buffers, by BD (3 mentions) Aurora cytometer, by FlowJo (3 mentions) Alexa fluor 488 conjugated mab against phosphorylated, by BD (2 mentions) Alexa fluor 647 conjugated mab against phosphorylated, by BD (2 mentions)

3 protocols using goat f ab 2 anti human iga g m

1

Phosphorylation of Syk and PLC-γ2 in BCR-stimulated PBMCs

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PBMCs stained with mAbs against CD19, CD20, CD3, CD27, CD21, IgD, and BV421-conjugated RBD-B.1 were resuspended in RPMI 1640–10% FBS and stimulated with anti-BCR as described previously (Kardava et al., 2018 (link)), with the following modifications. The cells were stimulated at 37°C for 2 min with 10 μg/ml goat F(ab’)2 anti-human IgA/G/M (# 109-006-064 from Jackson ImmunoResearch Laboratories). For the detection of phosphorylated signalling intermediates, cells were fixed and permeabilized using BD Cytofix and Phosflow Perm/Wash buffers (BD Biosciences) and stained with Alexa Fluor 488-conjugated mAb against phosphorylated Syk (p-Y348) and Alexa Fluor 647-conjugated mAb against phosphorylated PLC-2 (p-Y759) (BD Biosciences). The samples were acquired on an Aurora cytometer and analysis was performed by FlowJo V10.
Buckner C.M., Kardava L., Merhebi O.E., Narpala S.R., Serebryannyy L., Lin B.C., Wang W., Zhang X., de Assis F.L., Kelly S.E., Teng I.T., McCormack G.E., Praiss L.H., Seamon C.A., Rai M.A., Kalish H., Kwong P.D., Proschan M.A., McDermott A.B., Fauci A.S., Chun T.W, & Moir S. (2022). Recent SARS-CoV-2 infection abrogates antibody and B-cell responses to booster vaccination. medRxiv.
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2

Phosphorylation of Syk and PLCγ2 in BCR-Stimulated PBMCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs stained with mAbs against CD19, CD20, CD3, CD27, CD21, IgD, and BV421-conjugated RBD-B.1 were resuspended in RPMI 1640-10% FBS and stimulated with anti-BCR as described previously (Kardava et al., 2018 (link)), with the following modifications. The cells were stimulated at 37°C for 2 min with 10 μg/ml goat F(ab’)2 anti-human IgA/G/M (# 109-006-064 from Jackson ImmunoResearch Laboratories). For the detection of phosphorylated signaling intermediates, cells were fixed and permeabilized using BD Cytofix and Phosflow Perm/Wash buffers (BD Biosciences) and stained with Alexa Fluor 488-conjugated mAb against phosphorylated Syk (p-Y348) and Alexa Fluor 647-conjugated mAb against phosphorylated PLCγ2 (p-Y759) (BD Biosciences). The samples were acquired on an Aurora cytometer and analysis was performed by FlowJo V10.
Buckner C.M., Kardava L., El Merhebi O., Narpala S.R., Serebryannyy L., Lin B.C., Wang W., Zhang X., Lopes de Assis F., Kelly S.E., Teng I.T., McCormack G.E., Praiss L.H., Seamon C.A., Rai M.A., Kalish H., Kwong P.D., Proschan M.A., McDermott A.B., Fauci A.S., Chun T.W, & Moir S. (2022). Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses. Cell, 185(23), 4333-4346.e14.
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3

BCR Stimulation and Signaling Analysis

Cited 1 time
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PBMCs stained with mAbs against CD19, CD20, CD3, CD27, CD21, IgD, and BV421-conjugated RBD-B.1 were resuspended in RPMI 1640–10% FBS and stimulated with anti-BCR as described previously (Kardava et al., 2018 (link)), with the following modifications. The cells were stimulated at 37°C for 2 min with 10 μg/ml goat F(ab’)2 anti-human IgA/G/M (# 109-006-064 from Jackson ImmunoResearch Laboratories). For the detection of phosphorylated signaling intermediates, cells were fixed and permeabilized using BD Cytofix and Phosflow Perm/Wash buffers (BD Biosciences) and stained with Alexa Fluor 488-conjugated mAb against phosphorylated Syk (p-Y348) and Alexa Fluor 647-conjugated mAb against phosphorylated PLC-2 (p-Y759) (BD Biosciences). The samples were acquired on an Aurora cytometer and analysis was performed by FlowJo V10.
Buckner C.M., Kardava L., El Merhebi O., Narpala S.R., Serebryannyy L., Lin B.C., Wang W., Zhang X., Lopes de Assis F., Kelly S.E., Teng I.T., McCormack G.E., Praiss L.H., Seamon C.A., Rai M.A., Kalish H., Kwong P.D., Proschan M.A., McDermott A.B., Fauci A.S., Chun T.W, & Moir S. (2022). Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses. Cell, 185(23), 4333-4346.e14.
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