LCN2 protein concentration from hippocampal lysates and plasma was analyzed by enzyme- linked immunosorbent assay (ELISA) kit and standard (NGAL ELISA kit, ab199083, Abcam) as per manufacturer's instructions.
Ngal elisa kit
Manufactured by Abcam
Sourced in United States
The NGAL ELISA kit is a laboratory assay used to measure the concentration of neutrophil gelatinase-associated lipocalin (NGAL) in biological samples. NGAL is a protein that can serve as a biomarker for various conditions. The kit provides the necessary reagents and materials to perform the enzyme-linked immunosorbent assay (ELISA) technique to quantify NGAL levels.
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Lab products found in correlation
3 protocols using ngal elisa kit
1
LCN2 Protein Quantification in Hippocampus and Plasma
2
Effect of SMYA Decoction on Metabolic Markers
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After an 8-week intervention of SMYA decoction, blood was taken by removing eyeball after fasting for 8 h, and 6-hour urine was also collected. GLU, TC, TG, HDL, LDL, Scr, UREA, ALT, and AST of serum (some results were submitted as Supplementary Materials
3
Kidney Biomarker Measurement Protocol
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Serum creatinine levels were measured by standard enzymatic methods with an automated analyzer. Enzyme-linked immunosorbent assays (ELISA) were performed in duplicate using the NGAL ELISA kit (Abcam, Cambridge, MA, USA), the human TIM-1/KIM-1/HAVCR Quantikine ELISA kit (R&D System, Minneapolis, MN), the L-FABP human ELISA kit (Hycult, Biotech, Uden, The Netherlands), and the human IL-18 ELISA kit (Medical and Biological Laboratories, Nagoya, Japan). To standardize the biomarker measurement of perfusate sample, “post” concentration minus “base” concentration was defined as the final biomarker concentration used in our study.
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