The ADS051 inhibitory activity on the efflux of an MRP2 substrate, saquinavir (Sigma‐Aldrich), or an MDR1 substrate, talinolol (Toronto Research Chemicals, Toronto, ON, Canada), in a Caco‐2 monolayer system was investigated to evaluate ADS051 efficacy in an assay orthologous to the neutrophil transmigration studies described above. Caco‐2 cells (described earlier) were incubated with either 10 μm saquinavir alone, 10 μm saquinavir with 20 μm MK‐571 (control inhibitor; Sigma‐Aldrich), 10 μm talinolol alone, 10 μm talinolol with 100 μm haloperidol (Sigma‐Aldrich) or verapamil (Sigma‐Aldrich) 25 μm (control inhibitors), or 10 μm substrate (saquinavir or talinolol) with ADS051 at 25 μm , as shown in Fig. S1 . The involvement of efflux transporters was evaluated based on bidirectional permeability. Samples from the apical and basolateral sides at time zero and after 2 h of incubation at 37 °C were analyzed by LC‐MS/MS. Calculations were performed using peak area ratios.
Talinolol
Manufactured by LGC
Sourced in Canada
Talinolol is a laboratory equipment product manufactured by LGC. It is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. The core function of Talinolol is to provide efficient and reliable chromatographic separation, enabling researchers and analysts to accurately identify and quantify target analytes in their samples.
Automatically generated - may contain errors
Lab products found in correlation
Talinolol, by Merck Group (1 mentions)
Saquinavir, by Merck Group (1 mentions)
Haloperidol, by Merck Group (1 mentions)
Mk571, by Merck Group (1 mentions)
Verapamil, by Merck Group (1 mentions)
Saxagliptin, by LGC (1 mentions)
Vildagliptin, by LGC (1 mentions)
Rivaroxaban, by LGC (1 mentions)
Sitagliptin phosphate, by LGC (1 mentions)
14 c metformin, by Moravek Biochemicals (1 mentions)
2 protocols using talinolol
1
Evaluating ADS051 Inhibitory Activity on MRP2 and MDR1 Efflux Transporters
2
Quantification of Metformin and Antidepressants
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Unlabeled metformin was purchased from Wako Pure Chemical Industries (Osaka, Japan), and [ 14 C]metformin (100 mCi/mmol) was purchased from Moravek Biochemicals (Brea, CA, USA). Unlabeled almotriptan malate was purchased from Sigma (St. Louis, MO, USA).
Unlabeled alogliptin, naratriptan hydrochloride, rivaroxaban, saxagliptin, sitagliptin phosphate, sumatriptan succinate, talinolol and vildagliptin were purchased from Toronto Research Chemicals (Toronto, Ontario, Canada). All other chemicals and reagents were of analytical grade and are commercially available.
Unlabeled alogliptin, naratriptan hydrochloride, rivaroxaban, saxagliptin, sitagliptin phosphate, sumatriptan succinate, talinolol and vildagliptin were purchased from Toronto Research Chemicals (Toronto, Ontario, Canada). All other chemicals and reagents were of analytical grade and are commercially available.
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