Xenograft tumors and associated lungs were fixed in 4% paraformaldehyde and paraffin embedded. 5 μm sections were stained with haematoxylin and eosin (H&E) to assess tissue cellular morphology or were used for IHC after heat-induced antigen retrieval. Four paraffin embedded sections from each group (6 pmx and 7 pmx-LRIG1 tumors) were randomly chosen for H&E and LRIG1 IHC stain. The slides were incubated in 3% H2O2 to quench endogenous peroxidase activity, permeabilized with Triton-X100 in PBS, followed with blocking with 5% goat normal serum in PBS + 0.05% Tween (PBST). Anti-human LRIG1 (1:100, Genetex, USA cat#GTX119485) was used in 5% goat normal serum in PBST. The sections were incubated with biotinylated secondary antibody (Jackson Labs, USA) and Streptavidin-Peroxidase-HRP (Invitrogen, USA). For all slides, final detectable signal was visualized by NovaRED substrate kit (Vector lab, USA). After counterstaining with haematoxylin, slides were mounted.
Anti human lrig1
Manufactured by GeneTex
Sourced in United States
Anti-human LRIG1 is a primary antibody that specifically binds to the human LRIG1 protein. LRIG1 is a membrane-bound protein that regulates the activity of receptor tyrosine kinases. This antibody can be used for the detection and analysis of LRIG1 expression in various biological samples.
Automatically generated - may contain errors
2 protocols using anti human lrig1
1
Xenograft Tumor Histology and LRIG1 IHC
2
Xenograft Tumor Histology and LRIG1 IHC
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Xenograft tumors and associated lungs were fixed in 4% paraformaldehyde and paraffin embedded. 5 μm sections were stained with haematoxylin and eosin (H&E) to assess tissue cellular morphology or were used for IHC after heat-induced antigen retrieval. Four paraffin embedded sections from each group (6 pmx and 7 pmx-LRIG1 tumors) were randomly chosen for H&E and LRIG1 IHC stain. The slides were incubated in 3% H2O2 to quench endogenous peroxidase activity, permeabilized with Triton-X100 in PBS, followed with blocking with 5% goat normal serum in PBS + 0.05% Tween (PBST). Anti-human LRIG1 (1:100, Genetex, USA cat#GTX119485) was used in 5% goat normal serum in PBST. The sections were incubated with biotinylated secondary antibody (Jackson Labs, USA) and Streptavidin-Peroxidase-HRP (Invitrogen, USA). For all slides, final detectable signal was visualized by NovaRED substrate kit (Vector lab, USA). After counterstaining with haematoxylin, slides were mounted.
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