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Fluo 4 direct calcium assay buffer

Manufactured by Thermo Fisher Scientific
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Fluo-4 direct calcium assay buffer is a reagent used in fluorescence-based calcium detection assays. It is designed to be used with the Fluo-4 calcium indicator dye to measure intracellular calcium levels in a variety of cell types. The buffer provides a stable and optimized environment for the fluorescent dye to function effectively.

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Lab products found in correlation

Probenecid, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Fluo-4 (326 citations) Fluo-4 Direct (20 citations) Fluo-4 Direct™ Calcium Assay (10 citations)

2 protocols using fluo 4 direct calcium assay buffer

1

Calcium Flux and Cell Viability Assay

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1 × 105 cells were attached in 24 well plates. Media replaced with 500μL of 1X Fluo-4 direct calcium assay buffer supplemented with 5mM probenecid per manufacturer (Invitrogen) at 37C for 1.5 hours before addition of SYTOX and peptide. Live cell images acquired on AMG EVOS FL at 10X magnification using GFP and RFP channels. Fluor-4 and SYTOX intensity were measured in suspension cells at 4X magnification on Xcyto10, with fluorescence compensation, using 2-chamber slides. Time-lapse mean fluorescence intensity in live cells was acquired in 3-minute intervals of 4 individual fields of view at 20X magnification using CellInsight CY7 and GFP channels.
Eustace N.J., Anderson J.C., Warram J.M., Widden H.N., Pedersen R.T., Alrefai H., Patel Z., Hicks P.H., Placzek W.J., Gillespie G.Y., Hjelmeland A.B, & Willey C.D. (2020). A cell-penetrating MARCKS mimetic selectively triggers cytolytic death in glioblastoma. Oncogene, 39(46), 6961-6974.
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2

Calcium Flux and Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
1 × 105 cells were attached in 24 well plates. Media replaced with 500μL of 1X Fluo-4 direct calcium assay buffer supplemented with 5mM probenecid per manufacturer (Invitrogen) at 37C for 1.5 hours before addition of SYTOX and peptide. Live cell images acquired on AMG EVOS FL at 10X magnification using GFP and RFP channels. Fluor-4 and SYTOX intensity were measured in suspension cells at 4X magnification on Xcyto10, with fluorescence compensation, using 2-chamber slides. Time-lapse mean fluorescence intensity in live cells was acquired in 3-minute intervals of 4 individual fields of view at 20X magnification using CellInsight CY7 and GFP channels.
Eustace N.J., Anderson J.C., Warram J.M., Widden H.N., Pedersen R.T., Alrefai H., Patel Z., Hicks P.H., Placzek W.J., Gillespie G.Y., Hjelmeland A.B, & Willey C.D. (2020). A cell-penetrating MARCKS mimetic selectively triggers cytolytic death in glioblastoma. Oncogene, 39(46), 6961-6974.
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