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Bio a

Manufactured by Merck Group
Sourced in United States

BIO-A is a laboratory instrument designed for the analysis and quantification of biological samples. It utilizes advanced detection technologies to provide accurate and reliable data for researchers and scientists. The core function of BIO-A is to facilitate the measurement and analysis of various biomolecules, cellular components, and other biological analytes, enabling researchers to gain insights into complex biological systems.

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2 protocols using bio a

1

TLC-based Identification and Quantification of PCSEE and BIO-A

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PCSEE and BIO-A were concurrently run on a silica gel matrix (5 × 10 cm2) containing a fluorescent indicator with 254 nm excitation on aluminum foil (Sigma-Aldrich) for thin-layer chromatography (TLC) identification. On replicate TLC matrices, 5 μL of a 1 cm short band of 20 mg/mL PCSEE, and 1 and 5 mM BIO-A (Sigma-Aldrich) ethanol stock solution were directly deposited at 0.5 cm or more from TLC plate lower edge. After ethanol evaporation, TLC plate was slowly placed and developed in a clean glass developing chamber saturated with a miscible solvent system (water: acetone: ethanol of 5: 3: 1 ratio), 0.5 cm deep or less. Plates were taken out after 50-60 min incubation at RT, or when solvent front reached 1 cm from the upper TLC edge (approximately 8.5 cm height). Dried developed TLC plates were then visualized under short (254 nm) and long (366 nm) UV lights. Retention factor (Rf) of BIO-A band and a matching band were quantified.
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2

MAO-A and MAO-B Inhibition Assay

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The tested pure PCS constituents (≥95%) of PS and PSD and the standard selective MAO-AI clorgyline (CLORG) were obtained from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Hank's Balanced Salt Solution (HBSS), HEPES, benzylamine HCl, IPS, 6-PN, NBI, BIO-A, the selective MAO-BI standard selegiline (Deprenyl®) (DEP), and other chemicals and chromatography supplies were purchased from Sigma-Aldrich (St Louis, MO, USA). Human MAO-A and MAO-B, derived from a recombinant baculovirus infected insect cells, and measured in active units (U) were purchased from Sigma-Aldrich and received in a buffer solution at pH 7.4. The China originated PCS were obtained from East Earth Trade Winds (Redding, CA, USA), and the MAO-Glo™ kits from Promega (Madison, WI, USA).
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