A Zeiss Axio Observer D1 Inverted fluorescent microscope was used to capture live images. ZEN software, provided by Zeiss, was used to process the images.
Axio observer d1 inverted
Manufactured by Zeiss
The Axio Observer D1 inverted microscope from Zeiss is a versatile laboratory instrument designed for a variety of applications. It features an inverted optical configuration, allowing for the examination of samples from below. The microscope provides high-quality optics and illumination for clear and detailed imaging.
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3 protocols using axio observer d1 inverted
1
Automated Live Cell Imaging Workflow
2
Quantifying Cortical Cell Density
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14 μm thick cryosections were stained with antibodies, and images were acquired at 20X (Zeiss Axio Observer D1 inverted microscope). Counting was performed using FIJI (Image J) on 100 μm wide columns in the dorsal-lateral cortex in the region just anterior to the hippocampus.
3
Immunostaining of Sox2 in PMSCs
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PMSCs were fixed in 4% PFA in PBS prior to immunostaining, followed by membrane permeabilization with 0.5% Triton X-100 in PBS. Fixed cells were then incubated with monoclonal antibody for Sox2 (R&D Systems, MAB2018) overnight at 4 ° C. Cells were subsequently incubated with Alexa Fluor 488-or 546-conjugated secondary antibodies (Molecular Probes) for 1 h at room temperature. Cell nuclei were counterstained with DAPI, and images collected using a Carl Zeiss Axio Observer D1 inverted microscope.
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