Applied biosystems 3730 3730xl dna analyzer

DNA samples of the trio of family 1 (the proband and parents) were submitted for human identification (Macrogen). A short tandem repeat multiplex assay was performed with the AmpFISTR^®Identifiler^® KIT (Applied Biosystems, Foster City, CA, USA) using 15 tetranucleotide repeat loci and the Amelogenin gender-determining marker: all thirteen of the required loci for the Combined DNA Index System and two additional loci (D2S1338 and D19S433). Genotyping data were obtained with the Applied Biosystems^® 3730/3730xl DNA Analyzer and analyzed with GeneMapper ID v3.2 (Applied Biosystems).

The Sanger dideoxy chain termination technique on an Applied Biosystems 3730/3730Xl DNA Analyzer (Applied Biosystems, Foster City, CA, USA) by Bioneer Company was used for sequencing the PCR products. The primers used for sequencing were the same as PCR, except for C. trachomatis (Table I). Homology of the resultant sequences was checked using the NCBI-NIH BLAST search program.