TMEM43 (Myc-DDK-tagged)-Human transmembrane protein 43 (TMEM43) (NM_024334.2) was purchased from OriGene (RC200998), KCNK3 (Myc-DDK-tagged) (TASK-1) (NM_002246.3) was purchased from OriGene (RC215155), GJB2 (NM_004004) Human Tagged ORF Clone was purchased from OriGene (RC202092), and Cx30-msfGFP was purchased from Addgene (69019). The coding sequences of these genes were cloned into CMV-MCS-IRES2-EGFP or CMV-MCS-IRES2-dsRed plasmid vector using BglII/XmaI sites. TMEM43 truncation mutations were made using the EZchange site-directed mutagenesis kit (EZ004S, Enzynomics).
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