Samples were resolved on 4-12% Bis-Tris NuPAGE gels (Invitrogen) according to manufacturer’s recommendations and either stained with Commasie Blue (Bulldog Bio, Portsmouth, NH) or transferred to PVDF membrane for Western Blot staining using iBlot 2 Gel Transfer Device (Invitrogen). Western blot was performed using iBind Automated Western System (Invitrogen) according to manufacturer’s instructions using human anti-PD-L1 antibody (Avelumab, EMD Serona, 1 μg/ml) or rabbit monoclonal Na/K ATPase antibody (Abcam # ab76020, 1:100,000) as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch, 1:2000) or goat anti-rabbit IgG-HRP (Abcam # ab6721, 1:10,000) as sencondary antibodies. Membranes were developed using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA) and images were acquired using Amersham Imager 600 (GE Healthcare) with automated high dynamic range operation.
Commasie blue
Manufactured by Bulldog Bio
Coomassie Blue is a dye used in protein detection and quantification techniques. It binds to proteins and produces a blue color, allowing for the visualization and measurement of protein content in samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab6721, by Abcam (2 mentions)
Peroxidase conjugated donkey anti human igg hrp, by Jackson ImmunoResearch (2 mentions)
Amersham imager 600, by GE Healthcare (2 mentions)
Ibind automated western system, by Thermo Fisher Scientific (2 mentions)
Iblot 2 gel transfer device, by Thermo Fisher Scientific (2 mentions)
Nupage bis tris gel, by Thermo Fisher Scientific (2 mentions)
Clarity western ecl substrate, by Bio-Rad (2 mentions)
Ab76020, by Abcam (2 mentions)
Amersham imager, by Cytiva (2 mentions)
2 protocols using commasie blue
1
Western Blot Analysis of PD-L1 and Na/K ATPase
2
Western Blot Analysis of PD-L1 and Na/K ATPase
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Samples were resolved on 4-12% Bis-Tris NuPAGE gels (Invitrogen) according to manufacturer's recommendations and either stained with Commasie Blue (Bulldog Bio, Portsmouth, NH) or transferred to PVDF membrane for Western Blot staining using iBlot 2 Gel Transfer Device (Invitrogen). Western blot was performed using iBind Automated Western System (Invitrogen) according to manufacturer's instructions using human anti-PD-L1 antibody (Avelumab, EMD Serona, 1 µg/ml) or rabbit monoclonal Na/K ATPase antibody (Abcam # ab76020, 1:100,000) as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch, 1:2000) or goat anti-rabbit IgG-HRP (Abcam # ab6721, 1:10,000) as sencondary antibodies. Membranes were developed using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA) and images were acquired using Amersham Imager 600 (GE Healthcare) with automated high dynamic range operation.
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