The largest database of trusted experimental protocols

3 protocols using anti ndufs4

1

Immunohistochemical Analysis of Mitochondrial Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were from Sigma. Antibodies used included the following: anti-NDUFS4 (1:100 for immunohistochemistry [IHC] and 1:1,000 for western blot [WB]) was from Novus Biologicals (catalog no. NBP1-31465); anti-CD68 (1:100) was from Abcam (catalog no. ab125212); anti-GFAP (1:1,000) was from Sigma-Aldrich (catalog no. G3893); anti-NDUFA9, and anti-SDHB (1:1,000) were from MitoSciences (catalog no. ab14713). Secondary antibodies were from Promega (catalog nos. W4011 [rabbit] and W4021 [mouse]). PathoGreen histofluorescent stain (1,000X in water) was from Biotium (catalog no. 80027-5mL). The Novolink Polymer Detection System was from Leica Biosystems (catalog no. RE7140-K). The anti-LY6A antibody (dilution 1:1,000) was from Abcam (catalog no. ab109211).
+ Open protocol
+ Expand
2

Antibody Panel for Mitochondrial Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used included: anti-NDUFS4 (1:100 for immunohistochemistry and 1:1000 for western blot) was from Novus Biologicals (catalogue no. NBP1-31465); anti-CD68 (1:250) was from ABCAM; anti-GFAP (1:1000) was from Sigma; anti-NDUFA9, and anti- succinate dehydrogenase B (1:1000) were from MitoSciences (catalogue no. ab14713). Secondary antibodies were from Promega [catalogue nos. W4011 (rabbit) and W4021 (mouse)].
+ Open protocol
+ Expand
3

Mitochondrial Profiling Protocol: Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless stated otherwise, all chemicals were purchased from Sigma-Aldrich or ThermoFisher Scientific. The following antibodies were used: anti-β-actin (Novus Biologicals, Cat# NB600-501), anti-ATP5A (Abcam, clone 15H4C4, Cat# ab14748), anti-COX4 (Novus Biologicals, Cat# NB110-39115), anti-GAPDH (Protein Tech, Cat# 60004–1-Ig), anti-HSP60 (Santa Cruz, clone B-9, Cat# sc-271215 or Protein Tech, Cat# 15282–1-AP), anti-IQGAP1 (Santa Cruz, clone D-3, Cat# sc-374307 or clone C-9, Cat# sc-376021), anti-Lamin B1 (Santa Cruz, clone A-11, Cat# sc-377000), anti-Myc-Tag (Cell Signaling, clone 9B11, Cat#2276), anti-NDUFS4 (Novus Biologicals, Cat# NBP1-89026-25), anti-NDUFA4 (Santa Cruz, clone 2G7, Cat# sc-517091), anti-NDUFS1 (Santa Cruz, clone E-8, Cat# sc-271510), anti-NDUFAF2 (mimitin) (Santa Cruz, clone H-11, Cat# sc-365592), anti-SDHA (Santa Cruz, clone F-2, Cat# sc-390381), anti-UQCRC2 (Santa Cruz, clone G-10, Cat# sc-390378), anti-VDAC2 (Protein Tech, Cat# 11663-I-AP), HRP-conjugated goat anti-rabbit IgG (SouthernBiotech, Cat# 4050–05), HRP-conjugated goat anti-mouse IgG (SouthernBiotech, Cat# 1030–05), anti-rabbit-Alexa Fluor 555 (Molecular Probes, Cat# A27039), anti-rabbit-Alexa Fluor 594 (Molecular Probes, Cat# A21207), anti-mouse Alexa Fluor 488 (Molecular Probes Cat# A28175).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!