Anti‐human apoA‐I rabbit polyclonal antibody was obtained from ProteinTech. Anti‐human mast cell tryptase mouse monoclonal antibody was obtained from Chemicon International Inc. Anti‐human mast cell chymase (CC1) mouse monoclonal antibody and anti‐human β‐actin antibody were obtained from Abcam. Anti‐E‐cadherin rabbit monoclonal antibody, anti‐N‐cadherin rabbit monoclonal antibody, and anti‐vimentin rabbit monoclonal antibody were purchased from Cell Signaling Technology. Secondary biotin‐conjugated goat anti‐rabbit IgG antibody and goat anti‐mouse IgG antibody were obtained from Vector Laboratories.
Anti human β actin antibody
Manufactured by Abcam
Sourced in United States
Anti-human β-actin antibody is a primary antibody that binds to the β-actin protein, a widely expressed cytoskeletal protein found in eukaryotic cells. This antibody can be used to detect and quantify β-actin in various applications such as Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti vimentin rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti n cadherin rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti e cadherin rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
Qubit protein assay, by Thermo Fisher Scientific (1 mentions)
Ecl prime western blotting detection reagent, by GE Healthcare (1 mentions)
Blocking one, by Nacalai Tesque (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Bio-Rad (1 mentions)
3 protocols using anti human β actin antibody
1
Antibody Profiling for Cell Characterization
2
Quantitative Protein Analysis by Western Blot
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The concentration of total extracted protein was measured after 100-fold dH2O dilution by the Qubit Protein assay, in accordance with the manufacturer's instructions (Thermo Fisher Scientific). An equal amount (30 μg of protein) of lysate or culture medium was mixed with loading buffer, with this mixture then being electrophoretically separated and transferred onto membranes. The membranes were blocked with Blocking One (Nacalai Tesque, Kyoto, Japan), followed by incubation with anti-PD-L1 or MMP-7, -12, -13 antibodies (PD-L1, cat. no. 210931; MMP-7, cat. no. 205525; MMP-12, cat. no. 52897; MMP-13, cat. no. 51072; Abcam) and an anti-human β-actin antibody (cat. no. 4970; Cell Signaling Technology, Tokyo, Japan). After washing with Tris-buffered saline (TBS) with 0.05% Tween, the membranes were incubated with horseradish peroxidase-conjugated anti-mouse IgG. After washing with TBS-0.05% Tween-20, membranes were incubated with the ECL Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, UK). Signals were detected and analysed using C-DiGit (M&S TechnoSystems, Tokyo, Japan).
3
IL4I1 Protein Expression in MuSCs
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Western blotting was performed to measure the level of IL4I1 protein in MuSCs. Total protein was extracted using RIPA lysis buffer (Beyotime, Shanghai, China). Protein concentration was determined using a BCA protein assay kit (Bio-Rad, Hercules, CA, USA). 40 μg of protein was separated by a 10% SDS-PAGE gel and transferred to a polyvinylidene fluoride membrane (Millipore, Temecula, CA, USA). After blocking with 5% BSA for 2 h, the membrane was incubated with primary antibodies overnight at 4 °C. The primary antibodies included anti-human IL4I1 antibody (Cat# MAB5684, from R&D Systems, Minneapolis, MN, USA) and anti-human β-ACTIN antibody (Cat# ab6276, from Abcam). The membrane was then washed three times with TBST and respectively incubated with horseradish peroxidase (HRP)-conjugated anti-rat second antibody (Cat# 7077, from Cell Signaling Technology, Boston, MA, USA) and anti-mouse second antibody (Cat# ab205719, from Abcam) at room temperature for 1 h. Finally, the protein levels were detected by the chemiluminescence reagent.
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