Luria-Bertani broth and agar were purchased from Difco Laboratories (USA). Chemical re-agents were mainly purchased from Merck (Germany). Glacial acetic acid and ethanol (96%) were prepared from Mojallali co. (Iran). Kanamycin, ampicillin and anti-His tag antibody were purchased from Roche (Germany). Isopropyl β-D-1-thiogalactopyranoside (IPTG) was prepared from SinaClon (Iran). PCR master mix and 1 kb DNA Ladder were purchased from GoldBio(China).
Anti his tag antibody
Manufactured by Roche
Sourced in Germany
The Anti-His tag antibody is a laboratory reagent used for the detection and purification of recombinant proteins that have a histidine (His) tag. The antibody specifically binds to the His tag, allowing for the identification and isolation of the target protein from complex mixtures.
Automatically generated - may contain errors
2 protocols using anti his tag antibody
1
Bacterial Growth Media Preparation
2
ELISA for His-Tagged Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ELISA was conducted as previously described [23 (link)] with some
modifications. His-BL0675A was added to 96-well microtiter plates coated with PCMs and incubated at room
temperature for 1 hr. After washing three times with HBS-EP buffer (pH 7.4), an anti-His-tag antibody
(1:3,000; Roche) was added and the plates were incubated at room temperature for 40 min. After washing,
alkaline phosphatase-conjugated anti-mouse IgG (1:4,500; Dako, Glostrup, Denmark) was added for 40 min at room
temperature. Antibody-specific signals were detected with BluePhos MicroWell Substrate Kit (KPL, Gaithersburg,
MD, USA) by measuring the absorbance at 620 nm.
modifications. His-BL0675A was added to 96-well microtiter plates coated with PCMs and incubated at room
temperature for 1 hr. After washing three times with HBS-EP buffer (pH 7.4), an anti-His-tag antibody
(1:3,000; Roche) was added and the plates were incubated at room temperature for 40 min. After washing,
alkaline phosphatase-conjugated anti-mouse IgG (1:4,500; Dako, Glostrup, Denmark) was added for 40 min at room
temperature. Antibody-specific signals were detected with BluePhos MicroWell Substrate Kit (KPL, Gaithersburg,
MD, USA) by measuring the absorbance at 620 nm.
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