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A77 1726

Manufactured by Enzo Life Sciences
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The A77 1726 is a laboratory equipment product manufactured by Enzo Life Sciences. It is a technical device designed for use in scientific research and analysis, but a detailed factual description of its core function is not available without the risk of providing an extrapolated or biased interpretation.

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Lab products found in correlation

Leflunomide, by Merck Group (2 mentions) Progesterone, by Merck Group (2 mentions) Esomeprazole, by Enzo Life Sciences (2 mentions) Aphidicolin, by Enzo Life Sciences (2 mentions) A77 1726, by Merck Group (2 mentions) Uridine 5 monophosphate, by Merck Group (2 mentions) Cytidine 5 monophosphate, by Merck Group (2 mentions) Bongkrekic acid, by Merck Group (1 mentions) Cell culture media and supplements, by Thermo Fisher Scientific (1 mentions) Leflunomide, by Enzo Life Sciences (1 mentions) Cyclosporine a, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

3 protocols using a77 1726

1

Procurement of Small Molecule Compounds

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Leflunomide (≥98% purity) and A77 1726 (≥98% purity) were purchased from Enzo Life Sciences (Farmingdale, NY). Bongkrekic acid, cyclosporine A, and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO). Cell culture media and supplements were purchased from Life Technology (Grand Island, NY) and Atlanta Biologicals (Lawrenceville, GA).
Xuan J., Ren Z., Qing T., Couch L., Shi L., Tolleson W.H, & Guo L. (2018). Mitochondrial dysfunction induced by leflunomide and its active metabolite. Toxicology, 396-397, 33-45.
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2

Zebrafish Embryo Drug Screening

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Wild-type zebrafish embryos were treated with leflunomide from 50% epiboly until 24 hours post-fertilization in E3 embryo media and fixed with 4% Formaldehyde. Chemical libraries screened: LOPAC (n= 1280), ICCBL (n= 480) and NIH clinical collection (n=450). Other chemical used include leflunomide (Sigma L5025), Progesterone (Sigma, P0130), Esomeprazole (Enzo life sciences), Aphidicolin (Enzo life sciences, BML-CC101–0001) and A77 1726 (Enzo Life Sciences, ALX-430–096-M025). For nucleotide rescue experiments, a cocktail of 10 μg/ml uridine 5′-monophosphate (Sigma, UMP) and 10 μg/ml cytidine 5′-monophosphate (Sigma, CMP) was added to A77 1726. For in situ hybridizations, we followed the methods as described by Thisse C, and Thisse, B28 ,29 .
Santoriello C., Sporrij A., Yang S., Flynn R.A., Henriques T., Dorjsuren B., Greig E.C., McCall W., Stanhope M.E., Fazio M., Superdock M., Lichtig A., Adatto I., Abraham B.J., Kalocsay M., Jurynec M., Zhou Y., Adelman K., Calo E, & Zon L.I. (2020). RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells. Nature cell biology, 22(4), 372-379.
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3

Zebrafish Embryo Drug Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wild-type zebrafish embryos were treated with leflunomide from 50% epiboly until 24 hours post-fertilization in E3 embryo media and fixed with 4% Formaldehyde. Chemical libraries screened: LOPAC (n= 1280), ICCBL (n= 480) and NIH clinical collection (n=450). Other chemical used include leflunomide (Sigma L5025), Progesterone (Sigma, P0130), Esomeprazole (Enzo life sciences), Aphidicolin (Enzo life sciences, BML-CC101–0001) and A77 1726 (Enzo Life Sciences, ALX-430–096-M025). For nucleotide rescue experiments, a cocktail of 10 μg/ml uridine 5′-monophosphate (Sigma, UMP) and 10 μg/ml cytidine 5′-monophosphate (Sigma, CMP) was added to A77 1726. For in situ hybridizations, we followed the methods as described by Thisse C, and Thisse, B28 ,29 .
Santoriello C., Sporrij A., Yang S., Flynn R.A., Henriques T., Dorjsuren B., Greig E.C., McCall W., Stanhope M.E., Fazio M., Superdock M., Lichtig A., Adatto I., Abraham B.J., Kalocsay M., Jurynec M., Zhou Y., Adelman K., Calo E, & Zon L.I. (2020). RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells. Nature cell biology, 22(4), 372-379.
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