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Oxazepam glucuronide

Manufactured by Merck Group

Oxazepam-glucuronide is a chemical compound used in laboratory settings for research and analytical purposes. It is a metabolite of the benzodiazepine drug oxazepam. The compound can be utilized in various analytical techniques, such as chromatography and mass spectrometry, to study the pharmacokinetics and metabolism of oxazepam.

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2 protocols using oxazepam glucuronide

1

Synthetic Peptide Procurement and Reagents

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Synthetic light and heavy labeled peptides (Table S5) were obtained from New England Peptides (Boston, MA) and Thermo-Fisher Scientific (Rockford, IL), respectively. 17-β-estradiol, trifluoperazine, 4-hydroxyindole, propofol, naloxone, oxazepam, chloroform, Optima MS-grade acetonitrile/methanol and formic acid were purchased from Fischer Scientific (Fair Lawn, NJ). 17-β-Estradiol 3-glucuronide was purchased from Cayman chemicals (Ann Arbor, MI), whereas 17-β-Estradiol 17-glucuronide was purchased from Steraloid Inc. (Newport, RI). propofol β-D-glucuronide, naloxone 3β-D-glucuronide and oxazepam-glucuronide were purchased from Sigma Aldrich, Inc. (St. Louis, MO). Ammonium bicarbonate (98% purity) and sodium deoxycholate (98% purity) were obtained from Thermo Fisher Scientific (Rockford, IL) and MP Biomedicals (Santa Ana, CA), respectively.
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2

Warfarin Metabolism Enzyme Profiling

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The following chemicals and reagents were obtained from the sources shown: warfarin metabolites 4'-, 6-, 7-, 8-, and 10-hydroxywarfarin (Ultrafine Chemicals, Manchester); warfarin-sodium, ethanol, methanol, diethyl ether, ammonium acetate, acetic acid, K 2 HPO 4 , KH 2 PO 4 , Na 2 CO 3 , NaCl, NaOH, oxazepam, phenol reagent (Folin-Ciocalteu reagent) (Wako Pure Chemical Ind., Osaka, Japan); CuSO 4 5H 2 O was purchased from Kanto Chemicals (Tokyo, J -glucuronidase, carbamazepine, oxazepam glucuronide, phenyl-d5-7-hydroxywarfarin and racemic warfarin were purchased from Sigma-Aldrich Inc. (St. Louis, MO). Anti-rat CYP1A1, 2B1, 2C6, 2C11, and CYP reductase antibodies from goat and anti-rat CYP3A2 antibody from rabbit were purchased from Daiichi Pure Chemicals (Tokyo, Japan).
Anti-GAPDH antibody from mouse anti-goat IgG from donkey, anti-rabbit IgG from goat, and anti-mouse IgG from goat conjugated with horseradish peroxidase were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Heparin was purchased from Mochida Pharmaceutical CO., LTD (Tokyo, Japan). Midazolam ("Midazolam SANDOZ") was obtained from SANDOZ (Tokyo Japan). Butorphanol tartrate (Vetorphale) was obtained from Meiji Seika (Tokyo, Japan). Medetomidine (Dorbene p. 6
vet) was obtained from Kyoritsu Seiyaku (Tokyo, Japan). Isoflurane was obtained from DS Pharma Animal Health (Osaka, Japan).
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