NTA was performed on NanoSight LM14 system (NanoSight Technology, Malvern, Worcestershire, UK) equipped with a 532 nm green laser for determining the EV concentration and size distribution. Briefly, 10 μL separated EV samples were diluted with freshly 0.22 μm filtered PBS and loaded into the detection chamber by syringe. The same settings were used: camera level: 11, detect threshold: 14, capture: 60 s, number of capture: 5, temperature: 25 °C. The data were processed by NTA software (NTA version 3.3; Malvern Instruments, Malvern, Worcestershire, UK). A series of known numbers of 100 nm silica microspheres (Polysciences, Inc, catalog No: 24,041–10, Warrington, PA, USA) were used for the calibration of our measurement before using any EV samples. EV isolates from three kits were diluted to the optimum NTA detection range (1–10 × 108 particles/mL) before measurement.32 (link),33 (link) The same Nanosight parameters were used for all samples for a consistent comparison. The original particle concentrations from the isolates were then calculated based on the measured concentrations and the dilution factor.
Nta version 3
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The NTA version 3.3 is a lab equipment product from Malvern Panalytical. It is a particle analysis instrument that uses the technique of Nanoparticle Tracking Analysis (NTA) to measure the size and concentration of particles in liquid samples.
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Lab products found in correlation
2 protocols using nta version 3
1
Nanoparticle Tracking Analysis of EVs
2
Nanoparticle Tracking Analysis of Exosomes
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Nanoparticle tracking analysis (NTA) was performed on a NanoSight LM14 system (NanoSight Technology, Malvern, UK) equipped with a 532 nm green laser that could determine the exosome concentration and size distribution. A total of 100 µL of isolated exosome samples were diluted to 500 µL using freshly filtered PBS (0.22 µm filter) and loaded into the detection chamber with a syringe. The setting parameter of the camera was manually set and kept unchanged for all samples, with a slider shutter 650 and a slider gain of 50. Thirty-second videos were recorded, and the number of captures was 5. The detection threshold was set to 6, and the blur and max jump distance was automatically set. The temperature was maintained at 25 °C. The data were processed by the NTA software (NTA version 3.3; Malvern Instruments, Malvern, UK).
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