Total DNA was extracted from the plant leaves using the cetyltrimethylammonium bromide method (Allen et al., 2006 (link)). While total RNA (500 ng) was extracted using the EasyPure® Plant RNA kit (TransGen Biotech Co., Ltd, Beijing, China), reverse‐transcribed (RT) using a RevertAid First Strand cDNA synthesis kit (TransGen Biotech Co. Ltd) and quantified on an ABI Quantitative PCR Q6 Detection System (Thermo Fisher Scientific, Waltham, USA) with the SYBR Premix Ex Taq kit (TransGene Biotech Co., Ltd). The wheat actin gene was used as the reference gene, in which three independent biological replicates were conducted per analysis with at least three technical replicates. The primers have been listed in Table S6 .
Abi quantitative pcr q6 detection system
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
The ABI Quantitative PCR Q6 Detection System is a real-time PCR instrument designed for high-throughput quantitative analysis of DNA and RNA samples. It features a 96-well format and provides precise temperature control for reliable and reproducible results.
Automatically generated - may contain errors
2 protocols using abi quantitative pcr q6 detection system
1
Plant DNA and RNA Extraction Protocol
2
Extraction and Quantification of Plant Genetic Material
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Total DNA was extracted from the plant leaves using the cetyltrimethylammonium bromide (CTAB) method (Allen et al., 2006) . While total RNA (500 ng) was extracted using the EasyPure ® Plant RNA kit (TransGen Biotech Co., Ltd, Beijing, China), reverse-transcribed (RT) using a RevertAid First Strand cDNA synthesis kit (TransGen Biotech Co. Ltd, Beijing, China), and quantified on an ABI Quantitative PCR Q6 Detection System (Thermo Fisher Scientific, USA) with the SYBR Premix Ex Taq kit (TransGene Biotech Co., Ltd, Beijing, China). The wheat actin gene was used as the reference gene, in which three independent biological replicates were conducted per analysis with at least three technical replicates. The primers have been listed in Table S6.
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