Primary astrocytes (1 × 105) were resuspended in 200 μL FBS-free DMEM and seeded into the upper inserts (Corning). Media with microglia (MG) in each group was collected after 24 h of culture, then added in a volume ratio of 1:1 with complete DMEM in each of the lower layers, which underwent 24 h of culture. The astrocytes in the upper inserts were fixed with 4% paraformaldehyde (PFA; Servicebio, Wuhan, China) followed by staining with Crystal Violet reagent (KeyGen). We observed cells under the permeable membrane using a microscope (DM2000, Leica, Wetzlar, Germany).
Paraformaldehyde pfa
Manufactured by Wuhan Servicebio Technology
Sourced in China
Paraformaldehyde (PFA) is a polymer of formaldehyde used as a fixative in histology and microscopy. It is a white, crystalline solid that is soluble in water and other polar solvents. PFA is primarily used to preserve and fix biological samples, enabling their analysis and observation under a microscope.
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Lab products found in correlation
2 protocols using paraformaldehyde pfa
1
Astrocyte Migration in Microglia Co-culture
2
Transwell Migration Assay for HUVECs
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HUVECs (2 × 104/well) were seeded into the upper chamber of 8-µm pore-size, 24-well Transwell plates (Corning, NY, USA), and 200 μL serum-free ECM and 500 μL ECM complete medium were added into the upper and lower chambers, respectively. After 24 h, a cotton swab was used to remove unmigrated cells on the upper chamber surface. Migrated cells were fixed in 4% (w/v) paraformaldehyde (PFA; Servicebio, Wuhan, China), and then stained with 0.1% (w/v) crystal violet (Solarbio, Beijing, China). The migrated cells were counted under a light microscope (Nikon TE2000-E, Tokyo, Japan).
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