Au5800 automatic biochemical detector

Serum was collected after 2000 g centrifugation of blood at 4 °C for 15 min and was used to measure the levels of cytokines and serum parameters. Alanine transaminase (ALT), serum triglyceride (TC), total cholesterol (TG), and low-density lipoprotein cholesterol (LDL-C) levels were determined by the AU5800 Automatic biochemical detector (Beckman, CA, USA). The levels of interleukin (IL)-1β, IL-6, IL-10 and tumor necrosis factor α (TNF-α) in the liver were measured by ELISA kit (Mlbio, Shanghai, China). Spleen and thymus were weighed, and then spleen index and thymus index were calculated using the following equation:

The liver tissues were homogenized, and protein concentration was performed by the BCA Protein Assay Kit (NCM, Suzhou, China). The superoxide dismutase (SOD), malondialdehyde (MDA), and glycogen levels in the liver were measured by ELISA kit (Mlbio, Shanghai, China). The levels of aspartate aminotransferase (AST), ALT, albumin (ALB), and total protein (TP) were measured via an AU5800 Automatic biochemical detector (Beckman, CA, USA).