Isotype control clone mopc 21

Manufactured by BioXCell

Isotype control (clone MOPC-21) is a laboratory reagent used in flow cytometry and other immunological assays. It serves as a control to establish baseline signal levels and ensure the specificity of antibody binding. The MOPC-21 clone is a mouse IgG1 isotype control that does not recognize any known mammalian antigen.

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Lab products found in correlation

Anti ttr, by Abcam (1 mentions) Fitc isolectin b4, by Merck Group (1 mentions) Anti pdgfβr, by Abcam (1 mentions) Anti cd31, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Anti axl, by R&D Systems (1 mentions) Alexa fluor 488 conjugated goat anti rat igg h l, by Thermo Fisher Scientific (1 mentions) Cd 1 outbred mice, by Charles River Laboratories (1 mentions) Anti il 17a, by BioXCell (1 mentions) Anti il 17a clone 17f3, by BioXCell (1 mentions) D luciferin, by Biosynth (1 mentions)

Close spelling variants of this product

Anti-mouse IgG1 isotype control (clone MOPC-21) Mouse IgG1 isotype control antibody (clone MOPC-21) Mouse IgG1 isotype control (clone MOPC-21, Clone MOPC-21 MOPC-21 Isotype control

2 protocols using isotype control clone mopc 21

Immunofluorescence Assay for Flavivirus Antibodies

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Mouse-4G2 (monoclonal antibody against anti-flavivirus group antigen, clone D1-4G2-4-15, ATCC), Hu-4G2 (recombinant monoclonal antibody of clone D1-4G2-4-15 with human IgG Fc produced in Nicotiana tabacum, a gift from Dr. Nobuyuki Matoba), anti-TTR (Abcam), anti-CD31 (Invitrogen), anti- PDGFβR (Abcam), anti-alpha smooth actin (Abcam), anti-Axl (R&D systems), FITC-isolectin B4 (Sigma Aldrich), Alexa Fluor 647-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch), Alexa Fluor 549-conjugated goat anti-human IgG (Jackson ImmunoResearch), and Alexa Fluor 488-conjugated goat anti-rat IgG (H+L) (ThermoFisher) were used for the immunofluorescence assay. All antibodies were validated for their specificity using mock or isotype antibody controls.
Mouse IFNAR-1 neutralizing antibody (clone MAR 5A3) and isotype control (clone MOPC-21) were purchased from BioXcell. anti-Axl (AF154), anti-GAS6 (AB885), and isotype control IgG (AB-108-C) were purchased from R&D systems. The human type 1 IFN neutralizing antibody mixture was purchased from PBL Assay Science. Anti-Zika mouse IgM antibody (Clone ZKA185) and an isotype control mouse IgM antibody (anti-fluorescein, clone 4-4-20) were obtained from Absolute Antibody.

Kim J., Alejandro B., Hetman M., Hattab E.M., Joiner J., Schroten H., Ishikawa H, & Chung D.H. (2020). Zika virus infects pericytes in the choroid plexus and enters the central nervous system through the blood-cerebrospinal fluid barrier. PLoS Pathogens, 16(5), e1008204.

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Murine Toxoplasmosis Infection Model

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The luciferase-expressing PRU-Luc-GFP type II of T. gondii strain was provided by J. Boothroyd (Stanford University, Palo Alto, CA). Tissue cysts were obtained from the brains of CD1 outbred mice (Charles River) after 3 mo of infection with 20 cysts by i.p. injection. Experimental mice were infected with 5 cysts of T. gondii PRU-Luc-GFP orally. Weight was monitored every 48 h for the first 20 d and twice a week until day 50. Parasite burden was analyzed by bioluminescence measurements. Mice were imaged every 48 h for 20 d by i.p. injection of 150 mg of luciferin D (Biosynth AG) per kilogram of body weight and using a Xenogen IVIS 100 (Caliper Life Sciences). Data were analyzed with the Living Image Software (Caliper Life Sciences) and is expressed in relative light units. Antibody blockade was performed using anti–IL-17A (clone 17F3; Bio X Cell), isotype control (clone MOPC-21, Bio X Cell), and, in some experiments, anti–IL-17A and anti–IL-17F antibodies (provided by W. Ouyen, Genentech, South San Francisco, CA). For those experiments, mice were injected i.p. with 350 µg antibodies at days −1, 5, and 10 of T. gondii infection.

Cortez V.S., Cervantes-Barragan L., Song C., Gilfillan S., McDonald K.G., Tussiwand R., Edelson B.T., Murakami Y., Murphy K.M., Newberry R.D., Sibley L.D, & Colonna M. (2014). CRTAM controls residency of gut CD4+CD8+ T cells in the steady state and maintenance of gut CD4+ Th17 during parasitic infection. The Journal of Experimental Medicine, 211(4), 623-633.

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