Immunoblot analysis was carried out as described [52] (link), using antibodies against IE1 (6E1, Vancouver Biotech), IE2 (clone 12E2, Vancouver Biotech), GAPDH (clone 6C5, Santa Cruz), HA (clones 12CA5 and 3F10, Roche), Cyclin A2 (clone BF683, BD Biosciences), Cyclin B1 (clone GNS1, Santa Cruz), Cyclin D1 (clone EPR2241, Abcam), Cyclin E1 (clone H-12, Santa Cruz), CDK1 (PC25, Merck-Calbiochem), CDK2 (clone 55, BD Biosciences), APC5 (A301-026A, Bethyl Laboratories), Lamin A/C (clone 636, Santa Cruz), β-Tubulin (clone 2-28-33, Sigma-Aldrich), pp65 (clone CH-12, Santa Cruz), pp28 (clone 5C3, Santa Cruz), pp150 (clone XP1, a gift of Bodo Plachter). The Strep-tag HRP detection kit (IBA Lifesciences) was used to detect bacterially expressed Strep-pUL21a-His proteins.
Ha clones 12ca5 and 3f10
Manufactured by Roche
Sourced in United States
HA (clones 12CA5 and 3F10) is a set of monoclonal antibodies produced by Roche. These antibodies are designed to recognize and bind to the hemagglutinin (HA) protein, a surface protein found on influenza virus particles. The core function of these HA antibodies is to serve as tools for the detection and analysis of influenza virus samples in research and diagnostic settings.
Automatically generated - may contain errors
2 protocols using ha clones 12ca5 and 3f10
1
Immunoblot Analysis of Viral Proteins
2
Antibodies for SIRT1 Protein Analysis
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The following antibodies were used: mouse monoclonal SIRT1 7B7 (Novus Biologicals, LLC, Littleton, CO, USA) for immunofluorescence staining and mouse monoclonal SIRT1 Clone 3H10.2 (Millipore, Merck KGaA, Darmstadt, Germany), rabbit polyclonal SIRT1 (M07-131; Millipore, Merck KGaA) for immunoblotting, RPS19BP1 (AROS, ATLAS Antibodies AB, Stockholm, Sweden), PARP and p53 phospho-Ser46 (BD Pharming, Pharmingen, San Diego, CA, USA), p53 DO-1, GFP FL and PML H-238 (Santa Cruz Biotechnologies Inc., Dallas, TX, USA), Flag M2 (Sigma-Aldrich, St Louis, MO, USA), actin C4 (MP Biomedicals, (LLC, Santa Ana, CA, USA)), HA clones 12CA5 and 3F10 (Roche, F. Hoffmann-La Roche Ltd., Basel, Switzerland), p53 phospho-Ser46, SIRT1 (rabbit Ab), SIRT1 phospho-Ser27 (all Cell Signaling Technologies Inc., Danvers, MA, USA), acetyl-Lys373/382 p53 (Millipore, Merck KGaA). Affinity-purified rabbit HIPK2 antibodies have been described previously.33 (link), 39 (link) Phospho-specific SIRT1 pSer682 antibodies were generated by immunizing rabbits with the following KLH-coupled peptide: NH2-SGTCQ(pS)PSLEC-CONH2. Rabbit sera were affinity-purified against the phospho-peptide and subsequently non-phosphorylation specific antibodies were removed by a column containing the immobilized non-phospho-peptide. The rabbit SIRT1 phospho-Ser682 antibody will be commercially available soon from Millipore (Merck KGaA).
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