Ld plan neofluar 20 0.4 corr ph2

Green fluorescent protein-tagged histone H2B-expressing HeLa cells were seeded onto a six-well plate and transfected with siRNA. Twenty-four hours after transfection, two-channel time-lapse video microscopy was performed using a fully motorized Axiovert 200M microscope (Carl Zeiss, Oberkochen, Germany), equipped with AxiocamHRm. Temperature and CO₂ control was maintained using the Pecon Incubator (Houston, TX, USA), S-M and heating insert M06 controlled by Temp control (Erbach, Germany), 37-2 and CTI-Controller (Erbach, Germany), 3700. Both phase contrast and green fluorescent protein fluorescence images were acquired for 48 h with a lapse time of 5 min using AxioVision 4.3 software (Oberkochen, Germany). For routine and quantitative analyses, images were acquired using a × 20 objective lens (LD Plan-Neofluar × 20/0.4 Corr Ph2, Carl Zeiss).

HeLa cells were seeded onto a six-well plate and transfected with GFP-fusion constructs. Twenty-four hours after transfection, two-channel time-lapse video microscopy was performed using a fully motorized Axiovert 200 M microscope (Carl Zeiss), equipped with AxiocamHRm. Temperature and CO₂ control was maintained using the Incubator S-M and heating insert M06 controlled by Tempcontrol 37-2 and CTI-Controller 3700. Both phase contrast and GFP fluorescence images were acquired for 48 h with a lapse time of 5 min using AxioVision 4.3 software (Carl Zeiss). For routine and quantitative analyses, images were acquired using a × 20 objective lens (LD Plan-Neofluar × 20/0.4 Corr Ph2, Carl Zeiss).