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2 protocols using rabbit anti ets1

1

Antibody Utilization in Protein Analysis

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The following commercial antibodies were used: Mouse anti-EGFL7 (sc-373898, Santa Cruz, USA), Rabbit anti-EGFL7 antibody (ab115786, Abcam), mouse anti-Daxx (sc-8043, Santa Cruz), rabbit anti-Ets1 (sc-350, Santa Cruz), mouse anti-GAPDH (US Biological, USA), mouse anti-Flag M2 (Sigma Aldrich, USA) and mouse anti-Myc 9E10 (Sigma-Aldrich). Mouse anti-LANA monoclonal antibody was generated at Genscript (USA).
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2

Immunoblot Analysis of Apoptosis Regulators

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Actinomycin D, carbobenzoxy-Leu-Leu-leucinal (MG132), β-mercaptoethanol and mouse anti-β-tubulin antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-Ets-1, mouse anti-GFP and rabbit anti-ubiquitin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-caspase-3, rabbit anti-cleaved caspase-3 and horseradish peroxidase-conjugated anti-mouse or rabbit secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-HIF-1α antibody was purchased from Novus Biologicals (Cambridge, UK). Anti-rabbit light chain secondary antibody was purchased from Chemicon (Temecula, CA, USA).
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