Cd 1 a k a icr mice

All experiments were performed on male and female CD-1 (a.k.a. ICR) mice obtained from Charles River in age-matched cohorts of 5–6 weeks of age. All mice were recovered for at least 5 days after shipping prior to experimentation, and housed no more than 5 per cage. The animals were maintained in an AAALAC-accredited vivarium at the University of Arizona in temperature and humidity-controlled rooms on a 12-h light/dark cycle. Standard chow and water were available ad libitum. For all behavioral experiments, mice were brought to the testing room in their home cages for at least 30 min prior to the experiment for acclimation. Mice were randomized to treatment group by randomization of mice to cages upon arrival, and block random assignment of cages to treatment group. The experimenter was blinded to treatment group by the delivery of coded drug vials. The groups were not decoded until all data had been acquired. All experiments were approved by the University of Arizona IACUC, and were carried out in accord with the standards of the NIH Guide for the Care and Use of Laboratory Animals. We also adhered to the guidelines of ARRIVE; no adverse events were noted for any of the animals.