Log In Sign up
The largest database of trusted experimental protocols

Simponi

Manufactured by Johnson & Johnson
Visit Supplier

Simponi is a laboratory equipment product designed for general use in scientific research and analysis. It serves as a tool for performing various laboratory tasks and experiments. The core function of Simponi is to provide a reliable and efficient platform for conducting laboratory procedures.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Ms grade acetonitrile acn, by Thermo Fisher Scientific (1 mentions) Cho k1, by American Type Culture Collection (1 mentions) Sequencing grade trypsin, by Merck Group (1 mentions) Protease inhibitor tablet, by Merck Group (1 mentions) Lc ms grade formic acid, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions) Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Rituxan, by Genentech (1 mentions) Lc ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Halt protease phosphatase single use inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Protein deglycosylation mix 2, by New England Biolabs (1 mentions) Captureselect beads, by Thermo Fisher Scientific (1 mentions) Lc ms grade water, by Thermo Fisher Scientific (1 mentions) Bigdye terminator v3.1 cycle sequencing kit, by Thermo Fisher Scientific (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Humira, by Abbvie (1 mentions)

3 protocols using simponi

1

Golimumab Characterization and Cell Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Golimumab A and golimumab B were manufactured by Shanghai Zhangjiang Biotechnology Co., and purified by Protein A Sepharose (Shanghai Zhangjiang Biotechnology Co.). SIMPONI® (golimumab, Janssen) was obtained from the market of Shanghai, China. Acetonitrile (ACN, MS grade) was obtained from Thermo Scientific (United States); ammonium formate (NH4FA), ammonium bicarbonate (NH4HCO3), sodium cyanotrihydridoborate (NaBH3CN), fibrinopeptide (GFP), tris (2-carboxyethyl) phosphine hydrochloride (TCEP), and NaI were all obtained from Sigma (United States). Dithiothreitol (DTT), formic acid (FA, MS grade), and iodoacetamide (MIA) were obtained from Fluka (GER). Trypsin, IdeS (immunoglobulin-degrading enzyme of Streptococcus pyogenes), and recombinant human TNFα were obtained from Shanghai Zhangjiang Biotechnology Co. The CHO-K1 and the mouse fibroblastic cell line L929 cell lines were obtained from the American Type Culture Collection (ATCC).
Liu T., Xu J., Guo Q., Zhang D., Li J., Qian W., Guo H., Zhou X, & Hou S. (2022). Identification, Efficacy, and Stability Evaluation of Succinimide Modification With a High Abundance in the Framework Region of Golimumab. Frontiers in Chemistry, 10, 826923.
+ Open protocol
+ Expand
2

Antibody Formulations Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Golimumab formulation (Simponi, lot no. HJSV01, Janssen), infliximab formulation (Remicade, lot no. HIL49016P1, Janssen), adalimumab formulation (Humira, lot no. 1088193, Abbvie), and rituximab formulation (Rituxan, lot no. 3209283, Genentech) were purchased from Myoderm.
Diethylpyrocarbonate (DEPC), dimethyl sulfoxide (DMSO), Tris, iodoacetamide, sequencing-grade trypsin, and Protease Inhibitor Tablets were purchased from Sigma-Aldrich. LC-MS grade formic acid, LC-MS grade acetonitrile, LC-MS grade water, CaptureSelect beads, Bond-Breaker tris(2-carboxyethyl)phosphine (TCEP) solution neutral pH, Halt Protease & Phosphatase Single-Use Inhibitor Cocktail, DMEM high glucose (4 g/L) media, Opti-MEM, Lipofectamine 3000 transfection reagent, phosphate-buffered saline (PBS), benzonuclease, dodecyl-β-D-maltose (DDM), cholesteryl hemisuccinate tris salt (CHS), and fetal bovine serum (FBS) were purchased from ThermoFisher Scientific.
Protein Deglycosylation Mix II and the restriction enzymes KPN1 and XBA1 were purchased from New England Biolabs. HEK293T cells purchased from ATCC. mTNFα primers were purchased from IDT-DNA. BigDye Terminator v. 3.1 Cycle Sequencing Kit was purchased from Applied Biosystems.
Brenner D.A, & Frasier F. (1991). Cloning of murine ferrochelatase.. Proceedings of the National Academy of Sciences of the United States of America, 88(3), 849-853.
+ Open protocol
+ Expand
3

FDA-Approved TNF Inhibitors for Rheumatoid Arthritis

Check if the same lab product or an alternative is used in the 5 most similar protocols
We identified all self-administered TNF inhibitors approved by the FDA for the treatment of rheumatoid arthritis. Our sample included 4 products: etanercept (Enbrel; Amgen; approved in November 1998), adalimumab (Humira; AbbVie; approved in December 2002), golimumab (Simponi; Janssen Biotech; approved in April 2009), and certolizumab pegol (Cimzia; UCB; approved in May 2009).
San-Juan-Rodriguez A., Piro V.M., Good C.B., Gellad W.F, & Hernandez I. (2021). Trends in list prices, net prices, and discounts of self-administered injectable tumor necrosis factor inhibitors. Journal of Managed Care & Specialty Pharmacy, 27(1), 10.18553/jmcp.2021.27.1.112.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!