Ventana benchmark ultra slide stainer

The excision specimen from the left foot comprising skin and subcutaneous tissue was received in buffered neutral formalin. The tumor in the specimen was extensively sampled and processed for routine hematoxylin and eosin–stained sections. Five-μm sections from formalin-fixed, paraffin-embedded tissues were stained with antibodies against CD31 (Neomarker), CD34 (DAKO), ERG (Cell Marque), STAT6 (Cell Marque), Factor XIIIa (Cell Marque), CD163 (Cell Marque), desmin (DAKO), and SMA (DAKO) using the Ventana Optiview detection kit on the Roche Ventana BenchMark ULTRA slide stainer (Roche Diagnostics) after antigen retrieval, if required. For CD34, STAT6, Factor XIIIa, CD163, and desmin stains, the ULTRA cell conditioning method on the Roche Ventana BenchMark ULTRA slide stainer was used for antigen retrieval. For ERG and CD31 stains, the pressure cooker with citrate buffer at pH6 method was employed. For SMA, no antigen retrieval treatment was required.