Mouse monoclonal antibody c 219 against p gp

Elacridar and dithiothreitol (DTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), sterile phosphate-buffered saline (PBS), and penicillin/streptomycin antibiotics were obtained from Gibco (Grand Island, NY, USA). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Flowery Branch, GA, USA). Total protein measurement reagents using the bicinchoninic acid (BCA) method were obtained from Pierce (Rockford, IL, USA). Antibodies used were mouse monoclonal antibody against light chain LRP1 (Abcam, Cambridge, MA, USA), mouse monoclonal antibody C-219 against P-gp from BioLegend (San Diego, CA, USA), mouse monoclonal antibody against BCRP (Cell signaling; Boston, MA, USA). Monoclonal antibodies for claudin-5, ZO-1, and HRP-labeled secondary antibodies were purchased from Invitrogen (Carlsbad, CA, USA), goat polyclonal antibodies against actin (C-11) and Matrix Metallopeptidase 9 (MMP9) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies for pre- and postsynaptic proteins SNAP-25 and PSD-95, respectively, were purchased from GeneTex (Irvine, CA, USA), antibodies for IκB-α, p-IκB-α, NF-кB p65, and p-NF-кB p65 were purchased from Cell Signaling. All other reagents and supplies were purchased from VWR (West Chester, PA, USA) and Fisher Scientific (Hampton, NH, USA).