Estradiol was quantified at the Independent Public Health Care Center of the Ministry of the Interior and Administration and at the Warmia and Mazury Oncology Center in Olsztyn, Poland. The estradiol levels in the plasma were measured in an electrochemiluminescence immunoassay (ECLIA) with the use of Elecsys Progesterone II and Cobas e 411 analyzers (Roche, Basel, Switzerland). The radioimmunoassay (RIA) involved a commercial kit (ESTR-US-CT, producer CIS BIO ASSAYS), and it was based on a previously described procedure [44 (link)]. All the measurements were performed in duplicate for each cultured probe. The extraction yield for E2 was 90.67 ± 0.73%. The radioactivity of the samples with J125 was measured with an automatic Wallac 1470 WIZARDÆ gamma scintillation counter (Perkin Elmer, Waltham, MA, USA). The one-minute counts were performed with a Geiger counter (with a counting efficiency of 75%). The sensitivity of the assay for E2 was 1.36 pg/mL. The standard curve ranged from 2.72 to 550 pg/mL. The intra- and inter-assay coefficients of variation were 5% and 5%, respectively.
Elecsys progesterone 2
Manufactured by Roche
Sourced in Switzerland, Germany
The Elecsys Progesterone II is a laboratory diagnostic test used to measure the levels of progesterone, a hormone, in human blood or serum samples. It is designed to provide quantitative results for the assessment of progesterone levels.
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Lab products found in correlation
3 protocols using elecsys progesterone 2
1
Quantifying Estradiol Levels: Immunoassay Techniques
2
Feline Estrous Cycle and Pregnancy
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As the female cats attained puberty, there was exposure to a fertile tomcat during the estrous period. Matings were observed and/or diagnosed by the presence of spermatozoa in the vaginal smears. Twenty-one days after the end of estrus, blood samples were taken for ovulation assessment by electrochemiluminescence immunoassay determination of serum progesterone (Elecsys Progesterone II, Roche Diagnostics, Mannheim, Germany; P 4 > 5 ng/mL) and gestation was diagnosed by ultrasonic examination in all the females (Mattoon and Nyland, 1995) .
3
Serum Progesterone Profiling Post-FTAI
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On days 4, 7, 10, 13, 17 and 21 post FTAI, blood samples were collect by jugular venipuncture using vacuum tubes (BD Vacutainer ® , Franklin Lakes, NJ, USA). The samples were centrifuged for 15 min at 3000 rpm and the serum obtained was stored at À20 C until the time of analysis. Determination of serum P 4 concentrations was performed by chemiluminescence immunoassay (Elecsys ® , Progesterone II, Roche, Mannheim, Germany) with a sensitivity of 0.1 ng/ml, a specificity 90% and an intra-assay and inter-assay coefficient of variation < 10% for samples between 0.1 and 36 ng/ml.
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