15ug of fragmented biotinylated cRNA was hybridized to Gene Chip Human Genome U133 Plus 2.0 Array. Following standard conditions described in Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix, Santa Clarita, CA). Briefly, fragmented biotinylated cRNA was incubated with Gene Chip 133 plus 2.0 for 16 hours at 45oC before the hybridized chip was washed and stained in an Affymetrix fluidics station. The processed array was scanned, and the Q.C. was performed using Gene Chip Operating Software (GCOS, Affymetrix, CA). Same specimens performed by RNA-seq will be reported separately (although all data from single-cell RNA-seq will be presented by other manuals).
Gene chip 133 plus 2
Manufactured by Thermo Fisher Scientific
The Gene Chip 133 plus 2.0 is a microarray platform designed for gene expression analysis. It contains approximately 54,000 probe sets representing over 47,000 transcripts and variants, providing a comprehensive view of the human transcriptome.
Automatically generated - may contain errors
2 protocols using gene chip 133 plus 2
1
Genome-wide Transcriptome Analysis by Microarray
2
Genome-wide Transcriptome Analysis by Microarray
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15ug of fragmented biotinylated cRNA was hybridized to Gene Chip Human Genome U133 Plus 2.0 Array. Following standard conditions described in Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix, Santa Clarita, CA). Briefly, fragmented biotinylated cRNA was incubated with Gene Chip 133 plus 2.0 for 16 hours at 45oC before the hybridized chip was washed and stained in an Affymetrix fluidics station. The processed array was scanned, and the Q.C. was performed using Gene Chip Operating Software (GCOS, Affymetrix, CA). Same specimens performed by RNA-seq will be reported separately (although all data from single-cell RNA-seq will be presented by other manuals).
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