The largest database of trusted experimental protocols

2 5 diphenyl 3 4 5 dimethyl 2 thiazolyl tetrazolium bromide

Manufactured by Merck Group
Sourced in Brazil

2,5-Diphenyl-3-(4,5-dimethyl-2-thiazolyl) tetrazolium bromide is a chemical compound commonly used as a colorimetric indicator in various laboratory applications. It functions as a tetrazolium salt, which can be reduced to a colored formazan product. The core function of this compound is to serve as a reagent in colorimetric assays for the detection and quantification of cellular activity, metabolic processes, and other biological events.

Automatically generated - may contain errors

2 protocols using 2 5 diphenyl 3 4 5 dimethyl 2 thiazolyl tetrazolium bromide

1

Fabrication of Multifunctional Nanocarriers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tetraethylorthosilicate (TEOS), sodium periodate, sodium hydroxide (NaOH), 3-aminopropyl trimethoxysilane (APTES), cetyltrimethylammonium bromide (CTAB), dextrin (from maize starch) and ethylene glycol (EG) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Cystamine dihydrochloride (cystamine·2HCl, >98%) was purchased from Macklin Reagent Company. 2,5-Diphenyl-3-(4,5-dimethyl-2-thiazolyl) tetrazolium bromide (MTT) was purchased from Sigma-Aldrich. Doxorubicin hydrochlorides (DOX) were purchased from Sangon Biotech (Shanghai, China). All other chemicals were commercially available and used without further purification.
+ Open protocol
+ Expand
2

PLGA Nanoparticles with ACAT-Se Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols
Poly(d,l-lactic-co-glycolic acid) (PLGA, 50:50, 24–38 kDa), sorbitan monooleate (Span 80®), poloxamer 407 (Pluronic® F-127), 2-2-diphenyl-1-picrylhydrazyl (DPPH), radical, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,5-diphenyl-3-(4,5-dimethyl-2-thiazolyl) tetrazolium bromide (MTT), neutral red (NR) dye, phosphate-buffered saline (PBS), and trypsin-EDTA solution (0.5 g porcine trypsin and 0.2 g EDTA·4Na per liter of Hank’s Balanced Salt Solution) were obtained from Sigma–Aldrich (São Paulo, SP, Brazil). Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with L-glutamine (584 mg/L) and antibiotic/antimycotic (50 mg/mL gentamicin sulfate and 2 mg/L amphotericin B), were purchased from Vitrocell (Campinas, SP, Brazil). All other solvents and reagents were of analytical grade.
5′-Se-(phenyl)-3-(amino)-thymidine (ACAT-Se) was obtained from the LabSelen-NanoBio (Federal University of Santa Maria, Brazil). This compound was synthesized and fully characterized as previously described (da Rosa et al., 2017).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!