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2 protocols using accq fluortm pre column derivatization kit

1

Bioactive Amine and Amino Acid Analysis

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The reagents were of analytical grade, except high performance liquid chromatography -HPLC solvents. Ultrapure-water was from Milli-Q Plus (Millipore Corp., Milford, MA, USA). Bioactive amines (putrescine dihydrochloride, spermidine trihydrochloride, spermine tetrahydrochloride, agmatine sulfate, cadaverine dihydrochloride, serotonin hydrochloride, histamine dihydrochloride, tyramine hydrochloride, tryptamine, 2-phenylethylamine hydrochloride), ammonium chloride, and L-amino acids (aspartic acid, serine, asparagine, glycine, glutamic acid, glutamine, histidine hydrochloride, threonine, arginine hydrochloride, alanine, proline, cystine, tyrosine, valine, methionine, lysine hydrochloride, leucine, isoleucine, phenylalanine and norvaline) standards were from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). AccQ.FluorTM pre-column derivatization kit was from Waters (Milford, MA, USA). Analytical grade reagents were purchased from Merck (Rio de Janeiro, RJ, Brazil) and Hexis Científica (Jundiaí, SP, Brazil).
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2

Bioactive Amine and Amino Acid Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The reagents were of analytical grade, except high performance liquid chromatography -HPLC solvents. Ultrapure-water was from Milli-Q Plus (Millipore Corp., Milford, MA, USA). Bioactive amines (putrescine dihydrochloride, spermidine trihydrochloride, spermine tetrahydrochloride, agmatine sulfate, cadaverine dihydrochloride, serotonin hydrochloride, histamine dihydrochloride, tyramine hydrochloride, tryptamine, 2-phenylethylamine hydrochloride), ammonium chloride, and L-amino acids (aspartic acid, serine, asparagine, glycine, glutamic acid, glutamine, histidine hydrochloride, threonine, arginine hydrochloride, alanine, proline, cystine, tyrosine, valine, methionine, lysine hydrochloride, leucine, isoleucine, phenylalanine and norvaline) standards were from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). AccQ.FluorTM pre-column derivatization kit was from Waters (Milford, MA, USA). Analytical grade reagents were purchased from Merck (Rio de Janeiro, RJ, Brazil) and Hexis Científica (Jundiaí, SP, Brazil).
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