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Enhanced chemiluminescence ecl solution

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Enhanced chemiluminescence (ECL) solution is a reagent designed for the detection and quantification of proteins in Western blotting experiments. It generates a chemiluminescent signal when exposed to the target protein, which can be measured using a compatible imaging system.

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Lab products found in correlation

Hoechst 33258, by Merck Group (3 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Anti phospho jak2, by Cell Signaling Technology (1 mentions) Anti leptin, by Cell Signaling Technology (1 mentions) Anti stat3, by Cell Signaling Technology (1 mentions) Anti jak2, by Cell Signaling Technology (1 mentions) Anti phospho stat3, by Cell Signaling Technology (1 mentions) Pyrrolidine dithiocarbamate, by Merck Group (1 mentions) Pinacidil, by Cayman Chemical (1 mentions) Diazoxide, by Cayman Chemical (1 mentions) Anti p nf κb p65 antibody, by Cell Signaling Technology (1 mentions) Anti bax antibody, by Cell Signaling Technology (1 mentions) Anti bcl 2 antibody, by Cell Signaling Technology (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Mannitol, by Merck Group (1 mentions) Anti chop, by Cell Signaling Technology (1 mentions) Anti bip, by Cell Signaling Technology (1 mentions) Anti bax, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Enhanced chemiluminescence

4 protocols using enhanced chemiluminescence ecl solution

1

Cellular Signaling Pathway Analysis

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NRG, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1), dichlorofluorescein diacetate (DCFH-DH) and Hoechst 33258 were obtained from Sigma-Aldrich (St Louis, MO). Leptin antagonist (LA), leptin receptor antagonist (ObRa), and AG490 were purchased from ProSpec (East Brunswick, NJ). A Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies (Kumamoto, Japan). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco BRL (Grand Island, NY). Anti-STAT3, anti-phospho-STAT3, anti-JAK2, anti-phospho-JAK2, anti-leptin, and anti-leptin receptor antibodies were supplied by Cell Signaling Technology (Boston, MA), and horseradish peroxidase (HRP)-conjugated secondary antibody and bicinchoninic acid (BCA) protein assay kits were obtained from the KangChen Bio-tech, Inc. (Shanghai, China). Enhanced chemiluminescence (ECL) solution was purchased from the KeyGen Biotech (Nanjing, China).
Luo C., Ke X., Xiong S., Sun Y., Xu Q., Zhang W., Lei Y., Ding Y., Zhen Y., Feng J., Cheng F, & Chen J. (2019). Naringin attenuates high glucose-induced injuries and inflammation by modulating the leptin-JAK2/STAT3 pathway in H9c2 cardiac cells. Archives of Medical Science : AMS, 17(5), 1145-1157.
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2

Mitochondrial KATP Channel Modulation

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(NRG), 5,5′,6,6′-tetrachloro-1,1′,3,3′tetraethyl-imidacarbocyanine iodide (JC-1), Hoechst 33258, STZ and pyrrolidine dithiocarbamate (PDTC, a specific inhibitor of NF-κB pathway) were purchased from Sigma-Aldrich. Diazoxide (DZ, a mitochondrial K ATP channel opener), pinacidil (Pin, a non-selective K ATP channel opener), 5-hydroxydecanoic acid (5-HD, a mitochondrial K ATP channel blocker) and glibenclamide (Gli, a non-selective K ATP channel blocker) were purchased from Cayman Chemical. The cell counter kit-8 (CCK-8) was supplied by Dojindo Lab (Kumamoto, Japan). Fetal bovine serum (FBS) and DMEM medium were obtained from Gibco BRL; anti-kir6.1 (R-14) was purchased from Santa Cruz Biotechnology; anti-p-NF-κB p65 antibody, anti-p65 antibody anticaspase-3 antibody, anti-bax antibody and anti-bcl-2 antibody were purchased from Cell Signaling Technology; HRPconjugated secondary antibody and BCA protein assay kit were obtained from KangChen Bio-tech, Inc. (Shanghai, China). Enhanced chemiluminescence (ECL) solution was purchased from KeyGen Biotech (Nanjing, China).
You Q., Wu Z., Wu B., Liu C., Huang R., Yang L., Guo R., Wu K, & Chen J. (2016). Naringin protects cardiomyocytes against hyperglycemia-induced injuries in vitro and in vivo. The Journal of endocrinology, 230(2).
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3

Hydrogen Sulfide Signaling in Endothelial Cells

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Sodium hydrosulfide (NaHS; a donor of H2S) was purchased from Gibco-BRL (Grand Island, NY, USA). Fetal bovine serum (FBS), 2′, 7′-dichlorofluorescein diacetate (DCFH-DA), 740 Y-P (a PI3K agonist), LY294002 (a reversible PI3K inhibitor), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), M200 medium, D-glucose, Hoechst 33258 and mannitol were supplied by Sigma-Aldrich (St Louis, MO, USA). Cell counter kit-8 (CCK-8) was purchased from Dojindo Lab (Kumamoto, Japan). Anti-GAPDH (#8884), anti-ATF6 (#65880), anti-CHOP (#2895), anti-BiP (#3177), anti-phospho (p)-PI3K (#4228), anti-p-Akt (#4060), anti-p-eNOS (#9570), anti-total (t)-PI3K (#4249), anti-t-Akt (#4685), anti-t-eNOS (#9586), anti-Bax (#5023), anti-Bcl2 (#2827), anti-cleaved caspase 3 (#9661), anti-cleaved caspase 1 (#4199), anti-p-JNK(#4668), anti-t-JNK (#9252) and anti-gp91phox (#80897) antibodies were from Cell Signaling Technology (Boston, MA, USA). Enhanced chemiluminescence (ECL) solution was purchased from KeyGen Biotech (Nanjing, China). Interleukin (IL)-1β (#ab46052), IL-6 (#ab46027) and tumor necrosis factor (TNF)-α (#ab10054) ELISA kits were provided by Abcam (Cambridge, UK). Horseradish peroxidase (HRP)-conjugated secondary antibody and BCA protein assay kit were obtained from KangChen Bio-tech, Inc (Shanghai, China).
Lin F., Yang Y., Wei S., Huang X., Peng Z., Ke X., Zeng Z, & Song Y. (2020). Hydrogen Sulfide Protects Against High Glucose-Induced Human Umbilical Vein Endothelial Cell Injury Through Activating PI3K/Akt/eNOS Pathway. Drug Design, Development and Therapy, 14, 621-633.
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4

Mesenchymal Stem Cell Apoptosis Assay

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PGE1 was purchased from Zhuhai Schwarz Pharma Co., Ltd. (Zhuhai, China). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco Life Technologies (Carlsbad, CA, USA). Penicillin-streptomycin was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). A Hoechst 33258 Staining kit and propidium iodide (PI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Antibodies targeting Bcl-2 (cat. no. 2870), Bax (cat. no. 2772), and caspase-3 (cat. no. 9662), were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA) and were used at a dilution of 1:1,000. A Bicinchoninic Acid (BCA) Protein Assay kit was purchased from Kangchen Bio-tech (Shanghai, China). Enhanced Chemiluminescence (ECL) solution was purchased from KeyGen Biotech Co., Ltd. (Nanjing, China). Fluorescein isothiocyanate (FITC)-labeled Annexin V and anti-rat CD90 (cat. no. 11-0900-81), CD45 (cat. no. 17-0461-80), CD11b/c (cat. no. 12-0110-80), and CD29 (cat. no. 46-0291-80), antibodies were purchased from BD Biosciences (San Jose, CA, USA).
ZENG K., DENG B.P., JIANG H.Q., WANG M., HUA P., ZHANG H.W., DENG Y.B, & YANG Y.Q. (2015). Prostaglandin E1 protects bone marrow-derived mesenchymal stem cells against serum deprivation-induced apoptosis. Molecular Medicine Reports, 12(4), 5723-5729.
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