Mg 63

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in China

The MG-63 is a cell line derived from a human osteosarcoma, a type of bone cancer. It is widely used in cell culture research related to bone and skeletal tissues. The MG-63 cell line exhibits characteristics of osteoblast-like cells and is commonly employed to study bone cell biology, bone tissue engineering, and the evaluation of orthopedic biomaterials.

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83 protocols using mg 63

Lupeol Treatment of MNNG/HOS and MG-63 Cells

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MNNG/HOS and MG-63cells were bought from Cell Bank of the Chinese Academy of Sciences (Shanghai, China), MNNG/HOS and MG-63 cells were cultured as described previously [19 (link)], and cells were plated in 24-well plate for 24 h before transfection.
Moreover, Lupeol (Sigma-Aldrich, St. Louis, MO, USA) was re-suspended with ethyl alcohol to the concentration of 30 mmol/L mother liquor preparation. It would be diluted in dimethyl sulfoxide (DMSO; Solarbio, Beijing, China) at a 1:1 ratio. The final concentrations were diluted by cell culture medium for preparation.

Zhong J., He C., Xu F., Xu X., Liu L., Xu M., Guo Z., Wang Y., Liao J, & Li Y. (2020). Lupeol inhibits osteosarcoma progression by up-regulation of HMGA2 via regulating miR-212-3p. Journal of Orthopaedic Surgery and Research, 15, 374.

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Human Osteosarcoma Cell Line Cultivation

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Human osteosarcoma cell lines HOS, U-2OS, Saos-2, MG-63 and normal osteoblast cell NHOst were purchased from the Type Culture Collection of the Chinese Academy of Sciences. Cells were cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 units/ml penicillin and 100 mg/ml streptomycin). Cells were incubated at 37 °C in a humidified atmosphere of 5% CO2 in air. Antibodies against AKT2 and GAPDH were from Bioworld Technology (Atlanta, Georgia 30305, USA).

Liu Y., Zhu S.T., Wang X., Deng J., Li W.H., Zhang P, & Liu B.S. (2017). MiR-200c regulates tumor growth and chemosensitivity to cisplatin in osteosarcoma by targeting AKT2. Scientific Reports, 7, 13598.

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Osteosarcoma Cell Line Culture and Transfection

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Four human OS cell lines (SAOS-2, MG-63, U2OS, and HOS) and the normal human osteoblast hFOB1.19 cell line were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All these cell lines were incubated at 5% CO₂ and 37°C in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% of fetal bovine serum (FBS; both from Gibco, Invitrogen, Carlsbad, CA, USA) and 1% of a penicillin/streptomycin solution (Sigma‐Aldrich, St. Louis, MO).
The synthetic miR-618 mimics and miRNA mimic negative control (miR-NC) were purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, Guangdong, China). The MTDH-overexpressing plasmid was generated by inserting the MTDH cDNA lacking its 3′-UTR into the pCMV vector. This plasmid was chemically synthesized by Shanghai GenePharma Co., Ltd. (Shanghai, China). The small interfering RNA (siRNA) against MTDH (si-MTDH) was acquired from Qiagen GmbH (Hilden, Germany) and used to knock down endogenous MTDH expression. Negative control siRNA (si-NC) served as a control for si-MTDH. RNA oligonucleotides and the plasmid were transfected into cells using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA).

Li B., Zhao J., Zhao Q., Wu D., Zhang C., Zhao K., Song Y, & Gao C. (2019). MicroRNA-618 Directly Targets Metadherin mRNA To Suppress The Malignant Phenotype Of Osteosarcoma Cells By Reducing PTEN-AKT Pathway Output. OncoTargets and therapy, 12, 9795-9807.

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Osteoblast and Osteosarcoma Cell Culture

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The human normal osteoblast cell line NHost and several osteosarcoma cell lines (KHOS, 143b, LM7, U2OS, and MG-63) were obtained from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. All cells were cultured in Dulbecco's modified Eagle medium (DMEM) (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA) penicillin (100 U/ml), and streptomycin (100 μg/ml) at 37°C and 5% CO₂.

Zhou Q., Chen F., Zhao J., Li B., Liang Y., Pan W., Zhang S., Wang X, & Zheng D. (2016). Long non-coding RNA PVT1 promotes osteosarcoma development by acting as a molecular sponge to regulate miR-195. Oncotarget, 7(50), 82620-82633.

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Cell Line Cultivation and Treatment

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The cell lines MG-63, Saos-2 and hFOB 1.19 were purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai Institute of Cell Biology, China. The cells were cultured in DMEM (high glucose) supplemented with 10%FBS, 100 units/ml penicillin and 100 mg/ml streptomycin at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 ; for hFOB 1.19 cell culture, 0.3mg/ml G418 was also added, and the cells were cultured at 33.5°C. The cells treated with MLN2238 and DMSO (vehicle) in this study were all first 'starved' for 12h which means after cell adherence, complete medium was replaced with basal medium, followed by treatment with MLN2238 and DMSO dissolved in fresh basal medium. Additionally, the final DMSO concentration of MLN2238 (0-3.20µM) was much less than 0.1%, because the concentration of the stock solution of MLN2238 was 10mM.

Liu R., Fu C., Sun J., Wang X., Geng S., Wang X., Zou J., Bi Z, & Yang C. (2017). A New Perspective for Osteosarcoma Therapy: Proteasome Inhibition by MLN9708/2238 Successfully Induces Apoptosis and Cell Cycle Arrest and Attenuates the Invasion Ability of Osteosarcoma Cells in Vitro. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(2).

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Culturing Human Osteoblast Cell Lines

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Three human OS cell lines (143B, MG63 and SW1353) and normal human osteoblasts cell line (hFOB) and THP-1 cell line were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) medium supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) at 37°C under 5% CO₂.

Wang B., Wang X., Li P., Niu X., Liang X., Liu G., Liu Z, & Ge H. (2022). Osteosarcoma Cell-Derived Exosomal ELFN1-AS1 Mediates Macrophage M2 Polarization via Sponging miR-138-5p and miR-1291 to Promote the Tumorgenesis of Osteosarcoma. Frontiers in Oncology, 12, 881022.

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Osteosarcoma Cell Lines and Tissue Protocols

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Human OS cell lines HOS, U2OS, Saos-2, and MG-63 and normal human osteoblast (NHOst) cell were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai,China) and maintained in Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 ng/mL streptomycin. All cells were incubated at 37 C in an atmosphere of 5% CO 2 .
Osteosarcoma samples and the adjacent normal samples were collected from clinical patients undergoing OS resection. All these tissues were immediately snap-frozen in liquid nitrogen after surgery. This study was approved by the medical review board, and written informed consent was obtained from all patients.

Wang F., Yu D., Liu Z., Wang R., Xu Y., Cui H, & Zhao T. (2016). MiR-125b Functions as a Tumor Suppressor and Enhances Chemosensitivity to Cisplatin in Osteosarcoma. Technology in cancer research & treatment, 15(6).

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Osteosarcoma Cell Line Culture

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The MG-63 and U2-OS OS cell lines were purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai Institute of Cell Biology (Shanghai, China). The MG-63 and U2-OS cells were separately cultured in DMEM or McCoy's 5A medium with 10% FBS and 1% penicillin/streptomycin antibiotics. All the cells were maintained at 37°C in a humidified atmosphere of 95% air and 5% CO₂. The cells were treated with indicated concentrations of PI-1840 and DMSO (vehicle used as a control; the concentration of DMSO was <0.1%, which would not have affected the physiological status of the OS cells). The concentration of the stock solution of PI-1840 used was 40 mM (in DMSO).

Chen Y., Chen H., Xie H., Yuan S., Gao C., Yu L, & Bi Z. (2019). Non-covalent proteasome inhibitor PI-1840 induces apoptosis and autophagy in osteosarcoma cells. Oncology Reports, 41(5), 2803-2817.

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Culturing Human Osteosarcoma Cell Lines

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Human osteosarcoma cell lines MG-63, SAOS-2, U2OS, SW1353 and one osteoblastic cell line (hFOB) were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All osteosarcoma cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin and 100 g/ml streptomycin (Life Technologies, Grand Island, NY, USA) at 37 °C in 5% CO₂ and 95% air. Osteoblastic hFOB cells were grown in DMEM/F12 1:1 medium with 10% FBS, 2.5 mM L-glutamine and 0.3 mg/ml G418 at 37 °C in 5% CO₂ and 95% air. The cell lines passed the DNA profiling test (STR).

Li Q., Pan X., Wang X., Jiao X., Zheng J., Li Z, & Huo Y. (2017). Long noncoding RNA MALAT1 promotes cell proliferation through suppressing miR-205 and promoting SMAD4 expression in osteosarcoma. Oncotarget, 8(63), 106648-106660.

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Culturing Human Osteosarcoma and Osteoblast Cells

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Two human osteosarcoma cell lines (MG-63 and U2OS) and a normal human osteoblastic cell line hFOB were obtained from Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The hFOB cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). The human OS cells were cultured in RPMI-1640 medium supplemented with 10% FBS (both Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 mg/ml streptomycin (Thermo Fisher Scientific, Inc.). Cells were incubated at 37°C with 5% CO₂ in a humidified chamber.

Zhang H., Yan J., Lang X, & Zhuang Y. (2018). Expression of circ_001569 is upregulated in osteosarcoma and promotes cell proliferation and cisplatin resistance by activating the Wnt/β-catenin signaling pathway. Oncology Letters, 16(5), 5856-5862.

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