Lincomycin

All S. pseudintermedius isolates were tested for their antimicrobial susceptibility by disk diffusion method according to the guidelines of the French Society for Microbiology (CA-SFM ²). Antibiotics tested were cefovecin 30 μg (which was used as the phenotypic marker of methicillin resistance), penicillin 6 μg, fusidic acid 10 μg, kanamycin 30 μg, gentamicin 15 μg, tobramycin 10 μg, erythromycin 15 μg, spiramycin 100 μg, lincomycin 15 μg, tetracycline 30 μg, chloramphenicol 30 μg, florfenicol 30 μg, enrofloxacin 5 μg, vancomycin 30 μg, and teicoplanin 30 μg (Mast Diagnostics, Amiens, France). Bacteria were classified as susceptible, intermediate or resistant according to the clinical breakpoints approved by the veterinary part of the CA-SFM. Staphylococcus aureus ATCC 25923 was used as the quality control strain.

Antimicrobial susceptibility test was confirmed by the ANSES laboratory using the disk diffusion method and interpreted according to the guidelines of the Antibiogram Committee of the French Society for Microbiology³. S. aureus ATCC 25923 was used as quality control. In addition, 16 antibiotics of veterinary and/or human interest were tested: penicillin G, cefoxitin, cefovecine, kanamycin, gentamicin, tobramycin, tetracycline, erythromycin, spiramycin, lincomycin, chloramphenicol, florfenicol, fusidic acid, enrofloxacin, vancomycin, teicoplanin (Mast Diagnostics, Amiens, France).