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Alexa fluor 647 conjugated anti stat4

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Alexa Fluor® 647 conjugated anti‐STAT4 is a fluorescently labeled antibody that specifically binds to the STAT4 protein. STAT4 is a transcription factor involved in the signaling pathways of several cytokines. The Alexa Fluor® 647 dye allows for detection and analysis of STAT4 expression using techniques such as flow cytometry and immunofluorescence microscopy.

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Lab products found in correlation

Anti stat5, by BD (2 mentions) Alexa fluor 488 conjugated anti cd20 clone h1 fb1, by BD (2 mentions) Pe conjugated anti cd56 clone n901, by Beckman Coulter (2 mentions) Anti stat1, by BD (2 mentions) Anti p38 mapk, by BD (1 mentions) Anti nf κb p65, by BD (1 mentions)

Spelling variants of this product

Alexa Fluor 647 (58 citations) Alexa 647 (7 citations)

2 protocols using alexa fluor 647 conjugated anti stat4

1

Phospho-specific Antibodies for Multiparameter Flow Cytometry

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The following phospho‐specific monoclonal antibodies were used in 3 different panels during the flow cytometry protocol as described previously 34: Alexa Fluor® 647 conjugated anti‐STAT4 (pY693, clone 38/p‐STAT4, panel 1), anti‐STAT1 (pS727, clone K51‐856, panel 2) and anti‐STAT3 (pS727, clone 49/p‐STAT3, panel 3); PerCP‐Cy™5.5 conjugated anti‐ERK1/2 (pT202/pY204, clone 20A, panel 1), anti‐STAT1 (pY701, clone 4a, panel 2) and anti‐STAT3 (pY705, clone 4/P‐STAT3, panel 3); and PE‐Cy™7 conjugated anti‐NF‐κB p65 (pS529, clone K10‐895.12.50, panel 1), anti‐p38 MAPK (pT180/pY182, clone 36/p38, panel 2) and anti‐STAT5 (pY694, clone 47/ STAT5(pY694), panel 3) (all from BD Biosciences, San Jose, CA, USA). Cell surface markers incorporated in the assays were BV786 conjugated anti‐CD3 (clone SK7, BD HorizonTM), Alexa Fluor® 488 conjugated anti‐CD20 (clone H1 (FB1), BD Biosciences) and PE conjugated anti‐CD56 (clone N901, Beckmann Coulter, CA, USA).
Davies R., Hammenfors D., Bergum B., Vogelsang P., Gavasso S., Brun J.G., Jonsson R, & Appel S. (2018). Aberrant cell signalling in PBMCs upon IFN‐α stimulation in primary Sjögren's syndrome patients associates with type I interferon signature. European Journal of Immunology, 48(7), 1217-1227.
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2

Comprehensive Phospho-Protein Profiling by Flow Cytometry

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The following phospho-specific monoclonal antibodies were used in 3 different panels during the flow cytometry protocol described previously (17 (link)): Alexa Fluor®647 conjugated anti-STAT4 (pY693, clone 38/p-STAT4, panel 1), anti-STAT 1 (pS727, clone K51-856, panel 2), and anti-STAT3 (pS727, clone 49/p-STAT3, panel 3); PerCP-CyTM 5.5 conjugated anti-ERK1/2 (pT202/pY204, clone 20A, panel 1), anti-STAT1 (pY701, clone 4a, panel 2), and anti-STAT3 (pY705, clone 4/P-STAT3, panel 3); and PE-CyTM7 conjugated anti-p38 MAPK (pT180/pY182, clone 36/p38, panel 2), and anti NF-κB p65 (pS529, clone K10-895.12.50, panel 1), anti-STAT5 (pY694, clone 47/STAT5(pY694), panel 3) (all from BD Biosciences, San Jose, CA, USA). Cell surface markers incorporated in the assays were BV786 conjugated anti-CD3 (clone SK7, BD HorizonTM), Alexa Fluor® 488 conjugated anti-CD20 (clone H1 (FB1), BD Biosciences) and PE conjugated anti-CD56 (clone N901, Beckmann Coulter, CA, USA).
Davies R., Sarkar I., Hammenfors D., Bergum B., Vogelsang P., Solberg S.M., Gavasso S., Brun J.G., Jonsson R, & Appel S. (2019). Single Cell Based Phosphorylation Profiling Identifies Alterations in Toll-Like Receptor 7 and 9 Signaling in Patients With Primary Sjögren's Syndrome. Frontiers in Immunology, 10, 281.
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